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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
Quick-DNA Fecal/Soil Microbe Miniprep Kit
- 库存:
大量
- 供应商:
简石生物
- 保存条件:
常温
HIGHLIGHTS
- Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including from humans, birds, rats, mice, cattle, etc.
- Ultra-high density BashingBeads are fracture resistant and chemically inert.
- Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.
DESCRIPTION
The Quick-DNA Fecal/Soil Microbe Miniprep Kit is a fecal and soil DNA extraction kit designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA, which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
Miniprep/Kit-free high-throughput protocol
Background This protocol is adapted from "Molecular Cloning: A Laboratory Manual", Second Edition, Sambrook, Fritsch, and Maniatis. It is a quick, inexpensive way to purify large numbers of plasmids (I used to routinely do 80 at a time
Procedure for GenEluteTM Plasmid Miniprep Kit(质粒小量提取)
. 2.Lyse cells Lyse the resuspended cells by adding 200 µl of the Lysis Solution.Immediately mix the contents by gentle inversion (6-8 times)until the mixture becomes clear and viscous.Do not vortex.Harsh mixing wil shear genomic DNA ,resulting











