- 询价
- Cell Signaling Technology
- 3933
- USA
- 2025年12月31日
- W, IHC-P
- Rabbit
- H,M,R,Mk,Dm,X,Z,B,Pg,Hr
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Ubiquitin Antibody
- 抗原：
synthetic peptide corresponding to the amino terminus of the human ubiquitin protein
- 应用范围：
W, IHC-P
- 宿主：
Rabbit
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
H,M,R,Mk,Dm,X,Z,B,Pg,Hr
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster X=Xenopus Z=Zebrafish B=Bovine Pg=Pig Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | Source |
|---|---|---|---|
| W IHC-P | H M R Mk (Dm) (X) (Z) (B) (Pg) (Hr) | Endogenous | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Ubiquitin Antibody detects ubiquitin, polyubiquitin and ubiquitinated proteins. This antibody may cross-react with recombinant NEDD8. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of the human ubiquitin protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with the proteasome inhibitor MG132 (10 µM for 90 minutes), using Ubiquitin Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ubiquitin Antibody in the presence of control peptide (left) or antigen-specific peptide (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Ubiquitin Antibody. |
| Background | Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25a and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Studies of the Ubiquitin Proteasome System
) 6% to 8% SDS‐PAGE minigels (unit 6.1 ) Anti‐ubiquitin antibody ( protocol 31 ; for immunoblot detection) Boiling water bath Additional reagents and equipment for SDS
Several important signaling processes depend on the tagging of cellular proteins with “polyubiquitin chains”—ubiquitin polymers whose building blocks are connected by isopeptide bonds between G76 of one ubiquitin and a specific lysine residue
Measuring Ubiquitin Conjugation in Cells
Protein ubiquitination is crucial to many diverse and critical functions of cells. Although it has been long known that conjugation of ubiquitin to proteins results in their destruction by the proteasome, recently it has become apparent
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