- ￥1350 - 3000
- Proteintech
- 66516-1-Ig
- 2025年10月13日
- WB, IHC, ELISA
- Mouse
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, ELISA
- 靶点：
SMAD3
- 抗体英文名：
SMAD3 Monoclonal antibody
- 抗体名：
SMAD3 Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HT-1080 cells, NIH/3T3 cells, A431 cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells |
| Positive IHC detected in | human stomach cancer tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66516-1-Ig targets SMAD3 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rabbit |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SMAD family member 3 |
| 别名 | JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | NM_005902 |
| 基因名称 | SMAD3 |
| Gene ID (NCBI) | 4088 |
| RRID | AB_2881879 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P84022 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAKAuthors - Li Cui |
| human | WB | Drug Des Devel Ther Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 SignalingAuthors - Huan Hu |
| human | WB | Cancer Lett Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcomaAuthors - Qianyu Shi |
| human,mouse | WB | Cell Chem Biol Substrate-dependent interaction of SPOP and RACK1 aggravates cardiac fibrosis following myocardial infarctionAuthors - Wanqi Yang |
| mouse | WB,IF | Cell Mol Life Sci Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretionAuthors - Shiju Ye |
| human | CoIP | Diabetes Overexpressed Poldip2 incurs retinal fibrosis via the TGFβ1/SMAD3 signaling pathway in diabetic retinopathyAuthors - Zhiyu Ji |
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