- ￥1500 - 3280
- Proteintech
- 83609-5-RR
- 2025年10月09日
- WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human DMD protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
- 靶点：
Dystrophin
- 抗体英文名：
Dystrophin Recombinant monoclonal antibody
- 抗体名：
Dystrophin Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue, mouse skeletal muscle tissue, rat heart tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83609-5-RR targets Dystrophin in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag3408 Product name: Recombinant human DMD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 286-635 aa of BC028720 Sequence: YPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSDSMGEEDLLSPPQDTSTGLEEVMEQLNNSFPSSRGHNVGSLFHMADDLGRAMESLVSVMTDEEGAE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | dystrophin |
| 别名 | DMD, 240567A3, BMD |
| 计算分子量 | 3685 aa, 427 kDa |
| 观测分子量 | 430 kDa, 70 kDa |
| GenBank蛋白编号 | BC028720 |
| 基因名称 | Dystrophin |
| Gene ID (NCBI) | 1756 |
| RRID | AB_3671223 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P11532 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Front Pharmacol Cre recombinase affects calcium dynamics already in young miceAuthors - János Levin Liffers |
三句话读懂一篇 CNS：高糖饮食引起线粒体损伤；长期熬夜真的
-dCas9-p300core (dCas9p300) 和 Rosa26-LSL-dCas9-KRAB (dCas9KRAB)。利用该模型，Cre 重组酶能够诱导表达 dCas9p300 和 dCas9KRAB，进而精准调控机体基因表达和表观遗传修饰。图 5：来源 Nature Methods6. Immunity：硒缺乏导致 T 细胞分化紊乱的机制克罗恩病和溃疡性结肠炎是炎症性肠病的两个临床亚型。2021 年 8 月 2 日，浙江大学靳津教授团队在 Immunity 杂志上发表研究
)转移到小鼠成肌细胞C2C12中，hGH在小鼠肌肉和血浆中表达3个月以上，从而提出了成肌细胞途径基因治疗的设想。 目前已经有多种表达外源基因的小鼠成肌细胞直接注射小鼠中，可以获得持续表达，这些基因包括半乳糖苷酶基因、人凝血因子Ⅸ基因、多药耐药基因等。 1993年，Vincent等将含有人dystrophin小基因的重组腺病毒直接注射到患有Duchenne肌营养不良的mdx小鼠的肌肉内，可以在肌纤维中产生正常蛋白质达6个月，而且减轻了mdx小鼠的患病症状。
ml）lml，再从鸡的翼下静脉取血0.5--1ml，取出后放入刻度离心管中，再加入2--3ml Alsver液，使总量为4--5ml，混匀并封口，置于4℃冰箱内可供一周内使用，实验时取贮备的鸡血细胞lml，加入4ml85％生理盐水，混匀后以1200rr/min离心5min，去上清液后，最后用0.6mol/L的甘露醇液(或0.6mol/L蔗糖溶液)混匀后以上述离心条件洗涤两次，最后用0.6mol/L的甘露醇液将鸡细胞稀释并调整成每毫升含2×105个。如采用传代培养的骨髓或腹水瘤细胞，可先用0.9％
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