- 询价
- Cell Signaling Technology
- 4410
- USA
- 2025年09月11日
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- 详细信息
- 技术资料
- 抗体英文名：
Anti-mouse IgG (H+L), F(ab') Fragment (Alexa Fluor 647 Conjugate)__2__®
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 库存：
大量
- 供应商：
CST
- 标记物：
Alexa Fluor 647
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
250 ul/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 250 ul | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
Secondary Antibodies
Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4410
Specificity / Sensitivity
F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.
Flow Cytometry
Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb #2966 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) (green) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb #4545 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) (blue). Red = propidium iodide (fluorescent DNA dye).
High Content Screening
High content analysis of C2C12 cells exposed to varying concentrations of U0126 #9903 for 2 hours. With increasing concentrations of U0126, a significant decrease (~20 fold) in phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) signal as compared to the untreated control was observed. When using Phospho-p44/42 MAPK (Erk1/2) protein as a measurement, the IC50 of this compound was 2.5 μM. Data was generated on the Acumen® HCS platform using Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate).
Description
Anti-Mouse IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.
Directions for Use
The optimal dilution of the anti-species antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescent applications.
Applications
This product has been optimized for use as a secondary antibody in immunofluorescent applications.
Background
Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 4084 DRAQ5®
- 4753 Cellular Localization IF Antibody Sampler Kit
- 4408 Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
- 4409 Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
- 4412 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
- 4413 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
- 4414 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
- 4416 Anti-rat IgG (H+L), (Alexa Fluor® 488 Conjugate)
- 4417 Anti-rat IgG (H+L), (Alexa Fluor® 555 Conjugate)
- 4418 Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate)
- 5425 Normal Goat Serum
The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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