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Anti-rabbit IgG (H+L), F(ab') Fragment (Alexa Fluor 647 Conjugate)__2__®
详见说明书
CST
大量
详见MSDS文件
Alexa Fluor 647
0
-20°c
250 ul/carrier free & custom formulation / quantity
规格: | 产品价格: | ¥请询价 | |
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规格: | 250 ul | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951 and U0126 #9906 (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) compared to a nonspecific negative control antibody (red).
Confocal immunofluorescent analysis of mitotic HeLa cells using β-Tubulin (9F3) Rabbit mAb #2128 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) (blue) and Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #3475 (red).
High content analysis of A549 cells exposed to varying concentrations of LY294002 (#9901) for 3 hrs, followed by 100 ng/mL EGF for 20 minutes. With increasing concentrations of LY294002, a significant decrease (~5 fold) in phospho-S6 Ribosomal Protein (Ser235/236) signal as compared to the uninhibited control was observed. When using phospho-S6 as a measurement, the IC50 of this compound was 3.06 μM. Data were generated on the Acumen® HCS platform using Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate).
Anti-rabbit IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.
The optimal dilution of the anti-species antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescent applications.
This product has been optimized for use as a secondary antibody in immunofluorescent applications.
Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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