- 询价
- Cell Signaling Technology
- 3787
- USA
- 2025年12月25日
- IHC-P, IHC-F
- H,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-Akt (Ser473) (736E11) Rabbit mAb
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser473 of mouse Akt
- 应用范围：
IHC-P, IHC-F
- 库存：
大量
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 适应物种：
H,M,R
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (25 sections)/300 ul (75 sections)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (25 sections) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (75 sections) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IHC-P IHC-F | H M (R) | Endogenous | 60 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Akt (Ser473) (736E11) Rabbit mAb detects Akt1 only when phosphorylated at serine 473, and Akt2 and Akt3 only when phosphorylated at equivalent sites. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473 of mouse Akt. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and nuclear localization, using Phospho-Akt (Ser473) (736E11) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-Akt (Ser473) (736E11) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis using Phospho-Akt (Ser473) (736E11) Rabbit mAb on SignalSlide (R) Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Phospho-Akt (Ser473) (736E11) Rabbit mAb preincubated with an irrelevant peptide (left) or Phospho-Akt (Ser473) Blocking Peptide (#1140) (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using Phospho-Akt (Ser473) (736E11) Rabbit mAb (left) or PTEN (138G6) Rabbit mAb #9559 (right). MDA-MB-468 cells lack PTEN. Note the presence of P-Akt in the PTEN deficient cells. IHC-F (frozen)
Immunohistochemical analysis of frozen U-87MG xenograft, showing predominantly cytoplasmic localization using Phospho-Akt (Ser473)(736E11) Rabbit mAb. |
| Background | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
signaling的产品，诸如Akt总抗和Ser473（这两种抗体R&D的产品效价不错）。（特别注明：前几年CST的AKT抗体识别的条带在55KDa左右，而其他公司均认定为60KDa；最近还有战友提问这个抗体，重新检索了一下，应该是原AKT抗体（包括磷酸化）已经被CST自己下架了，从目前的几个抗体示意图看，大小也已与其他公司一致；因此，要不要买取决你自己）抗体公司在出售抗体的时候，在广告上会玩很多花样。A.不给整张膜标记的图示。很多抗体质量不好，背景高杂带多，如果靶蛋白区域较干净时，他们通常只给很窄的一条靶蛋白
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