TGF-β Antibody

3711:

Immunoprecipitation , Western Blotting

TGF-beta Antibody detects recombinant TGF-beta1, TGF-beta2, and TGF-beta3. The antibody also detects endogenous levels of the TGF-beta precursor proteins.

Polyclonal antibodies are produced by immunizing animals with synthetic peptide corresponding to a region in the carboxy terminus of TGF-beta1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of recombinant human TGF-beta1, TGF-beta2 and TGF-beta3, using TGF-beta Antibody.

Western Blotting

Western blot analysis of extracts from K562, 293 and Raji cells, using TGF-beta Antibody.

Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII) and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9).

There are three isoforms of TGF-beta designated TGF-beta1, TGF-beta2, and TGF-beta3, which are encoded by distinct genes and are expressed in a tissue specific manner (10). Each of the isoforms are synthesized as larger precursor proteins containing a propeptide region which gets cleaved prior to secretion from the cell. Mature TGF-beta contains two polypeptides linked linked by disulfide bonds forming a protein of about 25 kDa.

1. Massague, J. et al. (2000) Cell 103, 295-309.
2. Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402.
3. Derynck, R. et al. (2001) Nature Genet. 29, 117-129.
4. Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157.
5. Derynck, R. et al. (1997) Biochim. Biophys. Acta. 1333, F105-150.
6. López-Casillas, F. et al. (1991) Cell 67, 785-795.
7. Wang, X.F. et al. (1991) Cell 67, 797-805.
8. Derynck, R. et al. (1998) Cell 95, 737-740.
9. Massague, J. et al. (2000) Nat. Rev. Mol. Cell Biol. 1, 169-178.
10. Kingsley, D. M. et al. (1994) Genes Dev. 8, 133-146.

• Nishida, M. et al. (2008) EMBO J 27, 3104-15. Applications: Western Blotting

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• 3709 TGF-β (56E4) Rabbit mAb
• 3712 TGF-β Receptor I Antibody
• 2519 TGF-β Receptor III Antibody
• 3102 Smad2/3 Antibody
• 3101 Phospho-Smad2 (Ser465/467) Antibody
• 9520 Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.