Caspase-1 Antibody

2225:

IHC / Paraffin , Immunoprecipitation , Western Blotting

Caspase-1 Antibody detects endogenous levels of pro-caspase-1 and the caspase-1 p20 subunit. The antibody is expected to detect alpha, beta, gamma and delta isoforms.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues within the p20 subunit of human caspase-1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from THP-1 and HL-60 cells, using Caspase-1 Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Caspase-1 Antibody.

Caspase-1, or interleukin-1ß converting enzyme (ICE/ICEα), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1ß and interferon-γ inducing factor (IL-18) into their mature forms (1,2). Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis (3). Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1β and are resistant to endotoxic shock (4,5). At least six caspase-1 isoforms have been identified, including caspase-1 α, β, γ, δ, ε and ζ (6). Most caspase-1 isoforms (α, β, γ and δ) produce products between 30-48 kDa and induce apoptosis upon over-expression. Caspase-1 ε typically contains only the p10 subunit, does not induce apoptosis and may act as a dominant negative. The widely expressed ζ isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the α isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the "inflammasome" that includes caspase-5, Pycard/Asc, and NALP1 (7).

1. Thornberry, N.A. et al. (1992) Nature 356, 768-74.
2. Martinon, F. and Tschopp, J. (2004) Cell 117, 561-74.
3. Miura, M. et al. (1993) Cell 75, 653-60.
4. Kuida, K. et al. (1995) Science 267, 2000-3.
5. Li, P. et al. (1995) Cell 80, 401-11.
6. Feng, Q. et al. (2004) Genomics 84, 587-91.
7. Martinon, F. et al. (2002) Mol Cell 10, 417-26.

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• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8112 SignalStain^® Antibody Diluent

For Research Use Only. Not For Use In Diagnostic Procedures.