- 询价
- Cell Signaling Technology
- 4656
- USA
- 2025年08月09日
- W, F
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Cyclin A2 (BF683) Mouse mAb
- 抗原：
recombinant human cyclin A2 protein
- 应用范围：
W, F
- 适应物种：
H
- 供应商：
CST
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (8 western blots)/100 ul (20 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (8 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (20 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting F=Flow Cytometry
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W F | H | Endogenous | 55 | Mouse IgG2b |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Cyclin A2 (BF683) Mouse mAb detects endogenous levels of cyclin A2 and does not cross-react with other cyclins or Cyclin A1. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a recombinant human cyclin A2 protein. Western Blotting
Western analysis of extracts from Hela cells that were untreated, treated with doxorubicin (0.5 µM, 24 hours), or with nocodazole (50 ng/ml, 24 hours), using Cyclin A2 (BF683) Mouse mAb. Flow Cytometry
Flow cytometric analysis of untreated Jurkat cells, using Cyclin A2 (BF683) Mouse mAb versus propidium iodide (DNA content). Note decrease in expression of Cyclin A during late mitosis (arrow). |
| Background | While overcoming the G1/S checkpoint to commence DNA replication requires cyclin E, and traversing the G2/M checkpoint to initiate mitosis requires cyclin B to be present, cyclin A seems to be required for both S-phase and M-phase (1). A number of studies have described the ability of over-expressed cyclin A to accellerate the G1 to S transition causing DNA replication, and cyclin A antisense DNA can prevent DNA replication (2-4). Cyclin A availability is apparently the rate-limiting step for entry into mitosis, and cyclin A is required for completion of prophase (5). At late prophase, cyclin A may no longer be necessary as cdc2/cyclinB1 becomes active (5). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
-DNA-POD mAb. Mouse thymus apoptosis induced after in vivo treatment with dexametasone 21-phosphate. Stained apoptotic thymocytes (arrowheads, Fig. 1a) and macrophage with stained phagocytized apoptotic bodies in cytoplasm (arrowhead, Fig. 1b) are shown
beta-actin B-actin GAPDH(Human, rat and mouse) GAPDH(人/小鼠/大鼠 通用) GAPDH GAPDH/ NM_002046 GAPDH(大鼠、小鼠、人) β－actin(大鼠、小鼠、人) beta-actin beta-actin PCR or RT-PCR EBV BamHⅠ-W EBV Qp EBV Wp EBV Cp EBV Fp EBV LMP1 EBV LMP2A EBV
944-966 aaactcttactgctctccagt Human HeLa CHO1 (20) 138-156 AAGGUCAGUAAUACAACGGUG CHO corin, mouse (12) AACCACCGCCCUCCGUGCA HL-5 and 293 Cyclin B1 (5) J03166 199-217 CTCGTACAGCCTTGGGAGACA Xenopus embryo Cyclin B2 (5) J03167 252-270
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