Applications Key: W=Western Blotting Reactivity Key: H=Human M=Mouse Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
WIF1 Antibody detects transfected levels of total WIF1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln156 of human WIF1. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from COS cells, mock transfected or transfected with a mouse WIF1 construct, using WIF1 Antibody.
Background
WIF1 (Wnt inhibitory factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity (1). It contains an N-terminal WIF domain and five EGF-like repeats (2). The WIF1 ortholog in Drosophila, Shifted, is required for Hedgehog stability and diffusion (3,4). It has been reported that WIF1 expression is downregulated in many types of cancers (5-8).