- 询价
- Cell Signaling Technology
- 5063
- USA
- 2026年01月14日
- 免疫荧光（IF）,流式细胞（Flow Cyt）
- 兔
- 人,小鼠,大鼠,驴,其他,细菌,狗
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
α-Tubulin (11H10) Rabbit mAb (Alexa Fluor 488 Conjugate)
- 抗原：
/
- 应用范围：
免疫荧光（IF）,流式细胞（Flow Cyt）
- 宿主：
兔
- 库存：
大量
- 适应物种：
人,小鼠,大鼠,驴,其他,细菌,狗
- 供应商：
CST
- 抗原来源：
/
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Cytoskeletal Signaling
α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate) #5063
PhosphoSitePlus ® protein, site, and accession data: TUBA1B
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC F | H M R Mk Dm B (Dg) | Endogenous | Rabbit IgG |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster B=Bovine Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5063:
- Flow , Immunofluorescence
Specificity / Sensitivity
α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total α-tubulin protein and does not cross-react with recombinant β-tubulin.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human α-tubulin protein.
Flow Cytometry
Flow cytometric analysis of HeLa cells using α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate (green). Red = propidium iodide (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (11H10) Rabbit mAb #2125.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验LABELING ALPHA-ACTININ WITH IATR
.Dialyze overnight against buffer 5 in the cold room. 11.Clarify at 25,000 rpm,4℃,for 20 min in a 42.2Ti rotor. 12.Determine the concentration of alpha-actinin using Lowry assay.Determine dye/protein molar ratio by diluting conjugate 1:40 (10μl in 400μl
Antibody250µlIF-IC HCA4410SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4412SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F4413SAnti-rabbit IgG (H+L
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色（可选） 1. 将细胞重悬在 0.5 ml DNA 染色液中（例如 DRAQ5® #4084）。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗： 抗兔： Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4412 Anti-Rabbit IgG（H+L）， F（ab
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