【交流】目前已发表文章中RNAi设计的片段综述

Gene (Ref)
Accession #
Position from ATG
Gene Target
(Comments)
Cell Line Tested

ß-actin (1) NM_001101
972-994
aatgaagatcaagatcattgc
Human HeLa
gamma-actin (1) NM_001614
8-30
aagagatcgccgcgctggtca
Human HeLa
AIB1 (estrogen receptor coactivator) (22) AAAGACUCCUUAGGACCGCUU Ishikawa
Apaf-1 (31) 978-998 AATTGGTGCACTTTTACGTGA IMR90E1
ARC21 (1) AF006086
181-203
aagaatgaagctgataggacc
Human HeLa
ATR interacting protein (4) AF451323 254-274 AAGGTCCACAGATTATTAGAT Human HeLa
ATR interacting protein (4) AF451323 464-484 CTAGAGGAACAGAGAAGATCACA Human HeLa
ATR interacting protein (4) AF451323 867-887 AAGAAGAGGCCCAGAAAAGCT Human HeLa
Bcr-abl (32) AAGCAGAGUUCAAAAGCCCUU TonB, K562, hematopoetic cells
Brc-abl (32) AAAGCAGAGUUCAAAAGCCCU TonB, K562, hematopoetic cells
beta-tubulin- mouse neuronal (21) AF312873 AAGACAGAGCCAAGUGGACUC Mouse P19
Bruton's tyrosine kinase (Btk) (33) NM_000061 1682-1700 AAUUGGUAAACGAUCAAGGAG RBL-2H3, WEHI-231, NALM-6
Bruton's tyrosine kinase (Btk) (33) NM_000061 895-913 AAGGGAAAGAAGGAGGUUUCA RBL-2H3, WEHI-231, NALM-6
Bruton's tyrosine kinase (Btk) (33) NM_000061 518-536 AAGAAGCUUAAAACCUGGGAG RBL-2H3, WEHI-231, NALM-6
Casein Kinase I epsilon and delta (CKI-epsilon and CKI-delta) (17) 520-538 AACUGGGGAAGAAGGGCAACC HEK293
Caspase 1 (34) 191-211 AAGGGGCACAGGCATGCCAAA IMR90E1A
Caspase 2 (34) 94-114 AAACAGCTGTTGTTGAGCGAA IMR90E1A
hCdc2 (24) 125-147 HeLa
Cdc14A (23) 89-109 GCACAGTAAATACCCACTATT HeLa
cdk1 (1) NM_001786
125-147
aaggggttcctagtactgcaa
Human HeLa
CENP-E (1) NM_001813
944-966
aaactcttactgctctccagt
Human HeLa
CHO1 (20) 138-156 AAGGUCAGUAAUACAACGGUG CHO
corin, mouse (12) AACCACCGCCCUCCGUGCA HL-5 and 293
Cyclin B1 (5) J03166 199-217 CTCGTACAGCCTTGGGAGACA Xenopus embryo
Cyclin B2 (5) J03167 252-270 ATGTTAAGACTAAGCATGTAC Xenopus embryo
cytoplasmic dynein 1 heavy chain (1) U53530
509-531
aaggccaaggaggcgctggaa
Human HeLa
DIP13-alpha (DCC interacting protein) (25) AAGUGCAGCAACACACCUGAC DLD1
DIP13-beta (DCC interacting protein) (25) AAGCAGCACCUCUCCUCCCUU DLD1
Dishevelled (hDv-1 and hDv-3) (16)
AACAAGATCACCTTCTCCGAG A293

hDv-2 (16)
AACTTTGAGAACATGAGCAAC A293

Eg5 (1) NM_004523
1547-1569
aactggatcgtaagaaggcag
Human HeLa
emerin (1) NM_000117
628-650
aaccgtgctcctggggctggg
Human HeLa
eve (11) AACUCCUUGAACGGCAGCCGC Drosophila embryos
hFbw7 (cyclin E ubiquitination) (26) 713-735 see reference HeLa
Fortilin (35) 121-141 AAGGUAACAUUGAUGACUCGC U2OS
GalTII (galactosyltransferase II) (27) 65-87 AAGCACGCGACGCUGGCGCGC HeLa
GalTII (galactosyltransferase II) (27) 461-482 AAGGCGGACGACGACUCCUUC HeLa
GAS41 (1) NM_006530
327-349
aagacctgtaaccctgtatca
Human HeLa
Hec-1 (36) NM_006101 1517-1539 HeLa-S3
HPV E6 (38) AAGAGGUAUAUGACUUUGCUU CaSKi, SiHa, NDF, HCT116
HPV E7 (38) AAAGGAGGAUGAAAUAGAUGG CaSKi, SiHa, NDF, HCT116
HtrA2 (28) AAAACGGCUCAGGAUUCGUGG U2OS
HtrA2 (28) AAGGGGAGTTTGTTGTTGCCA U2OS
HtrA2 (28) AAGCTTTCCCGATGTTCAGCA
keratin 18 (1) NM_000224
1154-1176
aagatggcgaggactttaatc
Human HeLa
Kinase interacting stathmin (hKIS) (39) 160-180 AAGCAGTTCTTGCCGCCAGGA HEK293
Kinase interacting stathmin (mKIS) (39) 160-180 AAGCAGTTCCTGCCTCGGGGA HEK293
lamin A (13) NM019390
260-280 GAAGCAGCUUCAGGAUGAGAU Human HeLa S3
lamin A (19) AAGGACCUGGAGGUCUGCUG Hep3B
lamin A/C (1) NM_005572
608-630
aactggacttccagaagaaca
(90% reduction; 2)
Human HeLa
lamin B1 (1) NM_005573
672-694
aacgcgcttggtagaggtgga
Human HeLa
lamin B2 (1) M94362
1457-1479
aagaggaggaggaagccgagt
Human HeLa
LAP2 (1) NM_003276
37-59
aaagacaagttgaagagtgag
Human HeLa
Mad1 (41) AF083811 1843-1865 See reference HeLa-S3
Mammalian septin (MSF) (44) AF189712 472-492 See reference HeLa
Methyl-CpG-binding protein 2 (MeCP2) (19)
AAGCAUGAGCCCGUGCAGCCA Hep3B
Methyl-CpG binding domain protein 2 (MBD2) (19) AAGAGGAUGGAUUGCCCGGCC Hep3B
Microtubule-associated protein 2 (MAP2) (42) AAUCCUUCGUCUUUCCUCUCG primary neuronal cells
Myc-induced nuclear antigen 53 (mina53) (40) 45-63 See reference HeLa, MycB
MPS1 kinase (hMps1) (8) 133-155 See reference Human HeLa S3
Myeloid cell leukemia protein 1 (MCL1) (43) 456-476 AACCCGUCCGUACUGGUGUUA U2OS
Nicastrin (Nct) (45) AAGGGCAAGTTTCCCGTGCAG HEK293
NuMA (1) Z11583
3988-4010
aaggcgtggcaggagaagttc
Human HeLa
Nup153 (1) NM_005124
2593-2615
aaggcagactctaccaaatgt
Human HeLa
Origin recognition complex protein 6 (Orc6) (46) AAUGGUAGCCACAUCCGGUGU HeLa
Origin recognition complex protein 6 (Orc6) (46) AAGATTGGACAGCAGGUCGAC HeLa
P160 ROCK (47) 566-584; 639-657; 1958-1976; 2780-2798 See reference
Polo-like Kinase1 (hPlk1) (37) NM_005030 181-203 AAGGGCGGCTTTGCCAAGTGC HeLa, U2OS
Protein Kinase Receptor (PKR) (9) 931-949 AAGATCAAGTTTTGCCAATG Human HeLa
hRad9 (29) U53174 232-252 AAGUCUUUCCUGUCUGUCUUU MCF-7
Rad17 (7) AACAGACUGGGUUGACCCAUC Human HeLa
Signalling Adapter/scaffold protein (h/m Shc) (3) HSU73377 677-697 tcctacttggttcggtacatggg Human HeLa
p66 ShcA (3) HSU73377 236-256 CGGAATGAGTCTCTGTCATCGTC Human HeLa
Sphingosine kinase 1 (hSK1) (15)
70 nt downstream of start codon
AAGAGCUGCAAGGCCUUGCCC HUVEC
Steroid receptor coactivator (SRC-1) (30) CCUCAGGGCAGAGAACCAUCUTT Ishikawa
ß-tubulin (6) AF312873 3'-UTR GACAGAGCCAAGUGGACUCAC Mouse P19
Tissue Factor (hTF) (18) M16553
AAGCGCUUCAGGCACUACAAA HeLa, 293, Cos-1, HaCaT

Tissue Factor (hTF) (18) M16553
AAGAAGCAGACGUACUUGGCA HeLa, 293, Cos-1, HaCaT

ß-transducin repeat-containing protein (ß-TrCP), human (10) GUGGAAUUUGUGGAACAUCTT 293
TSG101 (a ubiquitin enzyme 2 variant protein) (14) 413-433 AACCUCCAGUCUUCUCUCGUC Human HeLa-M & HeLa KK3
VASP (1) XM_009141
1087-1109
aaagaggaaatcattgaagcc
Human HeLa
vimentin (1) NM_003380
346-368
aactacatcgacaaggtgcgc
(No silencing observed; 1,2)
Human HeLa
vimentin (1) NM_003380
1145-1167
aataccaagacctgctcaatg
Human HeLa
vimentin (1) NM_003380
863-885
aagaatggtacaaatccaagt
Human HeLa
vimentin (1) NM_003380
1037-1059
aaatggaagagaactttgccg
Human HeLa
vinculin, mouse (1) L18880
2923-2945
aagcggattagaaccaatctc
Mouse 3T3
zyxin, mouse (1) X99063
1357-1379
aaagcctaccacccacagtgc
Mouse 3T3


参考文献

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