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- 文献和实验
- 技术资料
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- 库存：
大量
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
- ATCC Number：
CRL-1896™
| Designations: | KM703 | ||
| Depositors: | PW Kincade | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against the functional domain of mouse Pgp-1 (CD44) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Comments: | Animals were immunized with the BMS2 bone marrow derived stromal cell line. Spleen cells were fused with Sp2/0 myeloma cells. The antibody weakly blocks adhesion of a B cell hybridoma (BM-2) to BMS2 cells or to hyaluronate coated dishes. The antibody prevents growth of lymphoid and myeloid cells in long term bone marrow cultures. KM703 recognizes an epitope unrelated to those detected by KM81, KM114 and KM201 (see ATCC TIB-241 , ATCC TIB-242 and ATCC TIB-240 ). |
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| Propagation: | ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15% |
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| Subculturing: | Medium Renewal: Every 2 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
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| References: | 1809: Miyake K, et al. Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures. J. Exp. Med. 171: 477-488, 1990. PubMed: 2406365 1811: Miyake K, et al. Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition. J. Exp. Med. 172: 69-75, 1990. PubMed: 2193100 |
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文献和实验on the gamma1, gamma2, gamma3, and alpha2 heavy chains (they are designated as G1m, G2m, G3m, and A2m allotypes, respectively), and on the kappa light chain (Km allotypes). Gm-Am allotypes are inherited in fixed combinations, or Gm-Am haplotypes, owing
酸性磷酸酯酶动力学性质分析 米氏常数（Km）和最大反应速度（Vm）的测定 [原理] 在温度、pH及酶浓度恒定的条件下，底物浓度对酶促反应的速度有很大的影响。在底物浓度很低时，酶促反应的速度（v）随底物浓度的增加而迅速增加；随着底物浓度的继续增加，反应速度的增加开始减慢；当底物浓度增加到某种程度时，反应速度达到一个极限值（Vmax
原理： 当环境的温度、pH和酶浓度等条件一定时，酶促反应的初速度(V)随底物浓度[S]增高而加快，直到达到一个极限，即最大反应速度(V)。底物浓度与酶反应初速度的这种关系，米一曼(Michaelis—Menten)氏推导出如下公式，称为米—曼氏方程式： 式中Km称为米式常数。Km是反应速度等于最大反应速度一半（v=V/2）时底物的浓度。Km是酶的特征性常数。测定Km是研究酶动力学的一种
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