immunoglobulin; monoclonal antibody; against p-nitroanaline amide derivatives
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Comments:
Animals were immunized with a conjugate of keyhole limpet hemocyanin and a phosphonamidate compound. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with and catalyzes the hydrolysis of certain ester bonds.
ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with:0.1 mM hypoxanthine0.032 mM thymidine0.05 mg/ml gentamicin10% fetal bovine serum Temperature: 37.0°C
Subculturing:
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.