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- 详细信息
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 库存：
大量
- 生长状态：
悬浮生长
- ATCC Number：
HB-9169™
| Designations: | P3 8D2 (SCRF 43.1) | ||
| Depositors: | Scripps Clinic and Research Foundation | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against p-nitroanaline amide derivatives | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with a conjugate of keyhole limpet hemocyanin and a phosphonamidate compound. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with and catalyzes the hydrolysis of certain ester bonds. |
||
| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with:0.1 mM hypoxanthine0.032 mM thymidine0.05 mg/ml gentamicin10% fetal bovine serum Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 21999: Lerner RA, et al. Molecules with antibody combining sites that exhibit catalytic properties. US Patent 5,126,258 dated Jun 30 1992 | ||
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P3 8D2 (SCRF 43.1)
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