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- 文献和实验
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 库存：
大量
- 生长状态：
悬浮生长
- ATCC Number：
HB-9169™
| Designations: | P3 8D2 (SCRF 43.1) | ||
| Depositors: | Scripps Clinic and Research Foundation | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against p-nitroanaline amide derivatives | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with a conjugate of keyhole limpet hemocyanin and a phosphonamidate compound. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with and catalyzes the hydrolysis of certain ester bonds. |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with:0.1 mM hypoxanthine0.032 mM thymidine0.05 mg/ml gentamicin10% fetal bovine serum Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 21999: Lerner RA, et al. Molecules with antibody combining sites that exhibit catalytic properties. US Patent 5,126,258 dated Jun 30 1992 | ||
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文献和实验恶性肿瘤病人外周血免疫指标的流式细胞仪检测及免疫治疗前后免疫功能变化的研究
2003年8月～2004年5月本院肿瘤科病房50例恶性肿瘤病人，均经病理确诊，其中肺癌23例，肝癌6例，乳癌4例，胃癌5例，胰腺癌2例，淋巴瘤4例，结肠癌3例，鼻咽癌1例，肾癌2例。男28例，女22例，年龄29～65岁，平均57岁。其中10例（肺癌6例，淋巴瘤4例）年龄29～53岁，平均41岁，经免疫增强剂（高聚生4ml静滴，每日1次，共3个月；白介素Ⅱ30万U皮下注射，每周2次，共3个月）治疗一疗程（3个月）后，复查一次。 对照组31例为健康体检者，男18例，女13例，年龄36～50岁，平均43岁
： 8g NaOH固体溶解于500mL双蒸水中，成0.4M NaOH溶液 10g SDS溶于500mL双蒸水中，成2% SDS溶液 使用前将上述两种溶液等体积混合后使用 注意： Buffer P2混合后不宜放置时间过场，每次配够一周使用的即可。长时间放置容易产生沉淀。若有沉淀产生，在37℃保温一段时间使沉淀溶解。 1.3 Buffer P3：（中和用） 成分：3M KAc（醋酸钾），pH4.8 配制：将147g KAc 溶于350mL双蒸水中，用约60
individually in plastic wrap. Take care to remove air bubbles between filter and wrap by pressing with a wipe to force out air. Place two filters in a large cassette (35x43cm), there will be a small overlap of filters but this normally is not a problem
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