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- 详细信息
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- 技术资料
- ATCC Number：
CRL-2123™
- 组织来源：
collecting duct
- 器官来源：
其他
- 运输方式：
冻存运输
- 细胞形态：
上皮样
- 库存：
大量
- 年限：
adult
- 生长状态：
贴壁生长
- 物种来源：
转基因小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
SV40转化细胞
| Designations: | mIMCD-3 | ||
| Depositors: | S Gullans | ||
| Biosafety Level: | 2 [CELLS CONTAIN PAPOVAVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus , transgenic deposited as mouse, transgenic | ||
| Morphology: | epithelial |
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| Source: | Organ: kidney, medulla Tissue: collecting duct Cell Type: SV40 transformed |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1991 | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Age: | adult | ||
| Comments: | mIMCD-3 is an inner medullary collecting duct (IMCD) cell line derived in 1991 by Michael Rauchman from a mouse transgenic for the early region of SV40 [Tg(SV40E)bri/7]. A tubule from the terminal one-third of the IMCD was microdissected and placed in culture. Confluent cells were subcultured and cloned using cloning cylinders. This is a polarized epithelia cell line which retains many differentiated characteristics of the terminal IMCD including inhibition of apical to basal sodium flux by amiloride and by atrial natriuretic peptide (ANP). The cells possess an amiloride sensitive sodium channel as determined by Western blot analysis, and accumulate the major organic osmolytes (inositol, sorbitol, betaine and glycerophosphorylcholine) in response to hypertonic stress. The cells secrete endothelin and form tubules and tight junctions. mIMCD-3 cells are responsive to Hepatocyte Growth Factor (HGF), and are readily adaptable to growth in hypertonic medium supplemented with NaCl and urea up to 910 mosmol/kg H20. These extreme osmotic conditions exist in the renal medulla in vivo, but are known to be lethal to most other cells. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 |
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| References: | 23528: Rauchman MI, et al. An osmotically tolerant inner medullary collecting duct cell line from an SV40 transgenic mouse. Am. J. Physiol. 265: F416-F424, 1993. PubMed: 8214101 | ||
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