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- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
B淋巴细胞
- 器官来源：
脾
- 免疫类型：
IgM
- 细胞形态：
淋巴样
- 库存：
大量
- 生长状态：
悬浮生长
- ATCC Number：
CRL-8753™
- 运输方式：
冻存运输
| Designations: | C5B7 | ||
| Depositors: | Miles Inc., Genetics Systems Corporation | ||
| Isotype: | IgM | ||
| Biosafety Level: | 2 [Cells Contain HERPESVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
||
| Source: | Organ: spleen Cell Type: B lymphocyte; Epstein-Barr virus (EBV) transformed |
||
| Cellular Products: | immunoglobulin; monoclonal antibody; against Pseudomonas aeruginosa lipopolysaccharide (LPS) of Fisher immunotype 1 (IATS type 6) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | B cells obtained from a patient with cystic fibrosis (whose serum contained above normal titer to P. aeruginosa) were transformed with Epstein-Barr virus (EBV). The antibody is specific for the lipopolysaccharide of the Fisher immunotype 1 of P. aeruginosa. |
||
| Propagation: | ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 85%; heat-inactivated fetal bovine serum, 15% Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
||
| Subculturing: | Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
||
| Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5% | ||
| Doubling Time: | 24 to 36 hrs | ||
| References: | 3862: Siadak AW, Lostrom ME. Human monoclonal antibodies to serotypic lipopolysaccharide determinants on Gram-negative bacteria and their production. US Patent 4,834,975 dated May 30 1989 | ||
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C5B7
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