- 询价
- 2025年11月22日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 生长状态：
悬浮生长
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- ATCC Number：
HB-9496™
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
| Designations: | XVI E6E6G10 | ||
| Depositors: | Wisconsin Alumni Res. Fndn. | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
||
| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against porcine (pig) receptor for 1,25-dihydroxy vitamin D3 | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Spleen cells were fused with NS-1 myeloma cells. The antibody can be used to assay 1,25-dihydroxy vitamin D. |
||
| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, 1 mM sodium pyruvate, 1 mM non-essential amino acids, 10 mM HEPES and 0.05 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15% |
||
| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
||
| Preservation: | fetal bovine serum, 95%; DMSO, 5% | ||
| References: | 1662: Dame MC, et al. Monoclonal antibodies to the porcine intestinal receptor for 1,25-dihydroxyvitamin D3: Interaction with distinct receptor domains. Biochemistry 25: 4523-4534, 1986. PubMed: 2429689 3923: Dame MC, et al. Assay for 1,25-dihydroxy vitamin D in sample containing vitamin D transport protein. US Patent 4,816,417 dated Mar 28 1989 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验红的是E6的开放读码，兰色的是E7的开放读码，中间有几个碱基，在基因组中E6和E7就是这样排列的， 请教各位高手，我如果简单的把上述E6E7的完整片段插到真核表达系统，能否做到E6E7的共同表达？如何分析，请指教 关于双基因的表达问题：我查了一些资料，里面很有学问。
In Vitro Assays of Substrate Degradation Induced by High-Risk HPV E6 Oncoproteins
The high-risk mucosal human papillomavirus E6 proteins were the first viral proteins that were shown to use the ubiquitin proteasome pathway for the inactivation of their cellular target proteins. The first substrate to be identified
Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene
A survey of the Medline titles for the years 2000–2001 revealed 125 papers whose titles included the name of the androgen-sensitive prostate cancer cell line LNCaP, and 36 papers also included PC3, the most common androgen independent line
技术资料暂无技术资料 索取技术资料
