- ￥1350 - 3000
- Proteintech
- 27309-1-AP
- 2025年10月13日
- IHC, IF/ICC, IF-P, FC (Intra), ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human KI67 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
IHC, IF/ICC, IF-P, FC (Intra), ELISA
- 靶点：
Ki-67
- 抗体英文名：
Ki-67 Polyclonal antibody
- 抗体名：
Ki-67 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue, human colon cancer tissue, human gliomas tissue, human lung cancer tissue, human skin cancer tissue, human lymphoma tissue, human breast cancer tissue, Insulinoma tissue, K-562 cells Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human lung cancer tissue |
| Positive IF/ICC detected in | HeLa cells, HEK-293 cells |
| Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27309-1-AP targets Ki-67 in IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, IF-P, FC (Intra), ELISA |
| 文献引用应用 | IHC, IF, FC (Intra) |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig, canine, zebrafish, hamster, goat |
| 免疫原 |
CatNo: Ag26266 Product name: Recombinant human KI67 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1201-1300 aa of NM_002417 Sequence: AGTLPGSKRQLQTPKEKAQALEDLAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRPKRSIRKADVEGELLACRNLMPSAGKAMHTPK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | antigen identified by monoclonal antibody Ki-67 |
| 别名 | KI67, MKI67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Ki 67 |
| 计算分子量 | 359 kDa |
| GenBank蛋白编号 | NM_002417 |
| 基因名称 | KI67 |
| Gene ID (NCBI) | 4288 |
| RRID | AB_2756525 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P46013 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.
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文献和实验| Species | Application | Title |
|---|---|---|
| IHC | Signal Transduct Target Ther FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growthAuthors - Wenxia Wei | |
| IHC | Mol Cancer lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2Authors - Jin Wang | |
| IHC | Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancerAuthors - Rashmi Bharti | |
| human | IHC | Cancer Discov Stress Granules determine the Development of Obesity-associated Pancreatic Cancer.Authors - Guillaume Fonteneau |
| IF | Adv Mater Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue RegenerationAuthors - Zhuo Li | |
| IHC | Protein Cell NDFIP1 limits cellular TAZ accumulation via exosomal sorting to inhibit NSCLC proliferationAuthors - Yirui Cheng |
。 3．胞浆内局限性点状阳性反应，如CDI$抗体的染色。 4．细胞膜线性阳性反应，大多数淋巴细胞标志物的染色均如此，如CD20。 5．细胞核阳性反应，如BrdU、Ki-67及雌、孕激素受体蛋白等。 有些抗原的阳性表达可同时出现在细胞的不同部位，如细胞质和细胞膜等。 《二》阳性染色结果定量 1．计算免疫反应阳性细胞数方法是选择每个样品中10个非重叠视野，计算每个视野中的阳性细胞与总细胞数的百分比，取平均数，连续观察和计算至少二个条件相同的样品
巢式 TD-PCR 扩增，扩增产物即为所需的DNA片段。在接头中，由于2条链中较短的一链的3’末端被 2NH2 封闭，使得引物 AP1 在第一次 PCR 之前没有结合位点，在 PCR 的第一循环过程中只能从 GSP1 延伸，没有 AP1 的延伸产物。而GSP1 的延伸产物产生了 AP1 的结合位点，在第二循环就开始从两端进行延伸反应。这样，就减少了非特异性扩增，从而提高了 PCR 的特异性.基因组步行由 2 轮 TD-PCR 反应组成。在 TD-PCR 的初始循环中，所采用的退火和延伸温度比引物
T-cell dependent antigen specific in situ staining (Pe)
: Double staining with TUNEL Double staining with antibodies Double staining (antibodies): 19- apply the diluted antibody (BLC-6, Ki-67, others) 20- wash twice in TBS-T 21- counterstain with an AP
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