相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human KI67 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
CoraLite®594 Fluorescent Dye
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
IF/ICC, FC (Intra)
- 靶点:
Ki-67
- 抗体英文名:
CoraLite®594-conjugated Ki-67 Polyclonal antibody
- 抗体名:
CoraLite®594-conjugated Ki-67 Polyclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | Ramos cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-27309 targets Ki-67 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 文献引用应用 | IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag26266 Product name: Recombinant human KI67 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1201-1300 aa of NM_002417 Sequence: AGTLPGSKRQLQTPKEKAQALEDLAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRPKRSIRKADVEGELLACRNLMPSAGKAMHTPK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | antigen identified by monoclonal antibody Ki-67 |
| 别名 | KI67, MKI67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Ki 67 |
| 计算分子量 | 359 kDa |
| GenBank蛋白编号 | NM_002417 |
| 基因名称 | KI67 |
| Gene ID (NCBI) | 4288 |
| RRID | AB_2919880 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P46013 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验| Species | Application | Title |
|---|---|---|
| human | IF | Oncol Rep Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancerAuthors - Liang Xu |
。 3.胞浆内局限性点状阳性反应,如CDI$抗体的染色。 4.细胞膜线性阳性反应,大多数淋巴细胞标志物的染色均如此,如CD20。 5.细胞核阳性反应,如BrdU、Ki-67及雌、孕激素受体蛋白等。 有些抗原的阳性表达可同时出现在细胞的不同部位,如细胞质和细胞膜等。 《二》阳性染色结果定量 1.计算免疫反应阳性细胞数方法是选择每个样品中10个非重叠视野,计算每个视野中的阳性细胞与总细胞数的百分比,取平均数,连续观察和计算至少二个条件相同的样品
【求助】免疫组化, FISH, CGH, LOH, MSI, 克隆性重排等的应用范围及应用”盲点”
性 (2)对疾病的预后和治疗的意义 (3)癌基因蛋白的应用 (4)对肿瘤增生程度的评价 ki67 PCNA (5)微小病灶的发现 微小癌 微小病灶(如羊水栓塞) (6)在肿瘤分期上的意义 (7)指导肿瘤治疗 (8)免疫性疾病的辅助诊断 (9)病原微生物的检测 应用“盲点”:由于抗体稀释度、多克隆抗体特异性问题、试剂盒选择、操作中时间温度染色过程等因素
R&D Systems支原体污染检测新方法(ELISA)专辑
MycoProbe®试剂盒的详细介绍 1. 试剂盒成分: 细胞裂解液 ,Cell Lysis Buffer 杂交板96孔板, Hybridization Plate 链亲和素捕获板96孔板, Streptavidin Capture Plate 样品稀释液 ,Sample Diluents 碱性磷酸酶标记的抗地高辛检测抗体 ,Anti-digoxigenin conjugated to alkaline phosphatase
技术资料暂无技术资料 索取技术资料










