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- 文献和实验
- 技术资料
- 免疫原:
Recombinant human S100A9 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC, IP, ELISA
- 靶点:
S100A9
- 抗体英文名:
S100A9 Polyclonal antibody
- 抗体名:
S100A9 Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | HL-60 cells, MCF-7 cells, THP-1 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue, mouse spleen tissue, human tonsillitis tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A549 cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26992-1-AP targets S100A9 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, sheep |
| 免疫原 |
CatNo: Ag25764 Product name: Recombinant human S100A9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-114 aa of BC047681 Sequence: KLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTP |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | S100 calcium binding protein A9 |
| 别名 | L1AG, CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B |
| 计算分子量 | 13 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC047681 |
| 基因名称 | S100A9 |
| Gene ID (NCBI) | 6280 |
| RRID | AB_2880716 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P06702 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.
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calcium binding protein A8 (S100A8) ELISA kit; CSB-EL020641MOMouse Protein S100-A9(S100A9) ELISA kit; CSB-EL020642MO 研究亮点 研究发现SFTSV可感染雄性小鼠生殖道(睾丸、附睾等),靶向间质细胞诱导凋亡和焦亡,降低睾酮水平并损害精子发生。单细胞测序揭示CCR2⁺/SPP1⁺巨噬细胞浸润驱动附睾炎症和纤维化,S100A4抑制剂氯硝柳胺可减轻病理损伤并降低死亡率。动物实验提示潜在性传播风险
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