- 询价
- 武汉三鹰Proteintech
- 26992-1-AP
- 武汉三鹰Proteintech
- 2025年07月15日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
北京云肽生物科技有限公司
- 抗体英文名：
S100A9 Polyclonal antibody
- 抗体名：
S100A9 Polyclonal antibody
- 浓度：
500 μg/ml
武汉三鹰Proteintech26992-1-AP S100A9 Polyclonal antibody
100 μL/50 μL/150 μL 浓度: 500 μg/ml
折扣低 发货快 有售后
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse And More (1)
Applications
IF, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
38
Cat No : 26992-1-AP
Synonyms
60B8AG, CAGB, Calgranulin B, Calprotectin L1H subunit, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP 14,
经过测试的应用
| Positive WB detected in | HL-60 cells, MCF-7 cells, THP-1 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue, mouse spleen tissue, human tonsillitis tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells, A549 cells, HepG2 cells |
推荐稀释比
| APPLICATION | DILUTION |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
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- 作者
- 内容
- 询问日期
文献和实验: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
Dual- and Triple-Color Fluorospot
stimulatory agents of choice and anti-CD28 (0.1 μg/ml; seeNote 4), in a final volume of 100–150 μl/well. The number of cells per well is dependent on the stimulating agent used (seeNote 5). b. Incubate the plate overnight at 37°C in a humidified
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