- ￥1350 - 3000
- Proteintech
- 15294-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human ADRP protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
Perilipin-2
- 抗体英文名：
Perilipin-2 Polyclonal antibody
- 抗体名：
Perilipin-2 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HepG2 cells, K-562 cells, mouse liver tissue, 3T3-L1 cells, NIH/3T3 cells, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, human renal cell carcinoma tissue, human liver tissue, human colon cancer tissue, human prostate cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | oleic acid treated HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15294-1-AP targets Perilipin-2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, canine, monkey, zebrafish, bovine, goat |
| 免疫原 |
CatNo: Ag7539 Product name: Recombinant human ADRP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 88-437 aa of BC005127 Sequence: DRIEERLPILNQPSTQIVANAKGAVTGAKDAVTTTVTGAKDSVASTITGVMDKTKGAVTGSVEKTKSVVSGSINTVLGSRMMQLVSSGVENALTKSELLVEQYLPLTEEELEKEAKKVEGFDLVQKPSYYVRLGSLSTKLHSRAYQQALSRVKEAKQKSQQTISQLHSTVHLIEFARKNVYSANQKIQDAQDKLYLSWVEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGVPQNIQDQAKHMGVMAGDIYSVFRNAASFKEVSDSLLTSSKGQLQKMKESLDDVMDYLVNNTPLNWLVGPFYPQLTESQNAQDQGAEMDKSSQETQRSEHKTH |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adipose differentiation-related protein |
| 别名 | ADFP, Adipophilin, Adipose differentiation-related protein, ADRP, ADRP/Perilipin 2 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 45-48 kDa |
| GenBank蛋白编号 | BC005127 |
| 基因名称 | Perilipin-2 |
| Gene ID (NCBI) | 123 |
| RRID | AB_2878122 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99541 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases. This antibody is not suitable for immunofluorescence staining of frozen sections.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | IF | Nature iPS-cell-derived microglia promote brain organoid maturation via cholesterol transferAuthors - Dong Shin Park |
| human | IF | Nature The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle.Authors - Simona Ricciardi |
| mouse | IHC | Signal Transduct Target Ther The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver diseaseAuthors - Chandana Thimme Gowda |
| mouse,human | IHC,IF | Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.Authors - Yi Fu |
| mouse | IF | Nat Commun Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagyAuthors - Donghai Cui |
| mouse | WB | Brain Behav Immun HIV-TAT dysregulates microglial lipid metabolism through SREBP2/miR-124 axis: Implication of lipid droplet accumulation microglia in NeuroHIVAuthors - Yan Cheng |
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