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SMAD2 多克隆抗体 12570-1-AP

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  • ￥1350 - 3000
  • Proteintech已认证
  • 12570-1-AP
  • 2025年10月13日
  • WB, IHC, IF/ICC, IP, ELISA
  • Rabbit
  • human, mouse, rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原：

      Recombinant human SMAD2 protein

    • 亚型：

      IgG

    • 保存条件：

      Store at -20°C. Stable for one year after shipment.

    • 克隆性：

      Polyclonal

    • 标记物：

      Unconjugated

    • 适应物种：

      human, mouse, rat

    • 保质期：

      详见产品包装

    • 库存：

      充足

    • 宿主：

      Rabbit

    • 应用范围：

      WB, IHC, IF/ICC, IP, ELISA

    • 靶点：

      SMAD2

    • 抗体英文名：

      SMAD2 Polyclonal antibody

    • 抗体名：

      SMAD2 Polyclonal antibody

    • 规格：

      50ul/100ul/150ul

    规格：50ul产品价格：￥1350.0
    规格：100ul产品价格：￥2200.0
    规格：150ul产品价格：￥3000.0

    经过测试的应用

    Positive WB detected inA549 cells, HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, HEK-293, HepG2 cells, MCF-7 cells, PC-3 cells, C6 cells, HEK-293 cells, HT-1080 cells, HUVEC cells, C2C12 cells
    Positive IP detected inHepG2 cells
    Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue, human endometrial cancer tissue, mouse colon tissue, rat colon tissue
    Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
    Positive IF/ICC detected inHepG2 cells
    Planning an IHC experiment? We recommend our IHCeasy SMAD2 Ready-To-Use IHC Kit. SMAD2 primary antibody included.

    推荐稀释比

    应用推荐稀释比
    Western Blot (WB)WB : 1:2000-1:10000
    Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
    Immunohistochemistry (IHC)IHC : 1:50-1:500
    Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
    It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
    Sample-dependent, Check data in validation data gallery.

    产品信息

    12570-1-AP targets SMAD2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

    经测试应用 WB, IHC, IF/ICC, IP, ELISA
    文献引用应用WB, IHC, IF, IP, CoIP, ChIP
    经测试反应性 human, mouse, rat
    文献引用反应性human, mouse, rat
    免疫原

    CatNo: Ag3237

    Product name: Recombinant human SMAD2 protein

    Source: e coli.-derived, PGEX-4T

    Tag: GST

    Domain: 1-355 aa of BC014840

    Sequence: MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAI
    宿主/亚型 Rabbit / IgG
    抗体类别 Polyclonal
    产品类型 Antibody
    全称 SMAD family member 2
    别名 JV18-1, JV18 1, JV18, hSMAD2, hMAD-2
    计算分子量 467 aa, 52 kDa
    观测分子量 52-70 kDa
    GenBank蛋白编号BC014840
    基因名称 SMAD2
    Gene ID (NCBI) 4087
    RRIDAB_2193037
    偶联类型 Unconjugated
    形式Liquid
    纯化方式Antigen affinity purification
    UNIPROT IDQ15796
    储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
    储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

    背景介绍

    SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SMAD2. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934). The ubiquitination form of Smad2 is ~70 kDa (PMID: 25998442).

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    相关实验

    • 有适合免疫沉淀IP的GST抗体吗？

      因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞

    • 抗体选择

      实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。   （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。   （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免

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    文献支持
    SMAD2 多克隆抗体 12570-1-AP
    ￥1350 - 3000