- ￥1350 - 3000
- Proteintech
- 11308-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), ELISA
- Rabbit
- human, canine
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human GM130;GOLGA2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, canine
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), ELISA
- 靶点：
GOLGA2/GM130
- 抗体英文名：
GOLGA2/GM130 Polyclonal antibody
- 抗体名：
GOLGA2/GM130 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, human spleen tissue, HEK-293 cells, MCF-7 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, MDCK cells, HEK-293 cells, HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11308-1-AP targets GOLGA2/GM130 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, canine |
| 文献引用反应性 | human, monkey, zebrafish, hamster |
| 免疫原 |
CatNo: Ag1848 Product name: Recombinant human GM130;GOLGA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-678 aa of BC014188 Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | golgi autoantigen, golgin subfamily a, 2 |
| 别名 | GM130, GOLGA2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, GM130;GOLGA2 |
| 计算分子量 | 111 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC014188 |
| 基因名称 | GM130 |
| Gene ID (NCBI) | 2801 |
| RRID | AB_2115327 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q08379 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)
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| WB | Nature Gasdermin D-mediated metabolic crosstalk promotes tissue repairAuthors - Zhexu Chi | |
| human | IF | Nature ER proteins decipher the tubulin code to regulate organelle distributionAuthors - Pengli Zheng |
Immunity The nucleotide receptor STING translocates to the phagosomes to negatively regulate anti-fungal immunityAuthors - Tian Chen | ||
| human | IF | Nat Methods Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).Authors - Marine H Laporte |
| human | IF | Adv Mater Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue RegenerationAuthors - Zhuo Li |
| WB | Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.Authors - Xiao Wang |
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