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- 文献和实验
- 技术资料
- 保存条件:
2-25℃
- 保质期:
5年
- 英文名:
NON-Fat Powdered Milk
- 库存:
999
- 供应商:
北京索莱宝科技有限公司
- 规格:
10g/100g/500g
| 规格: | 10g | 产品价格: | ¥40.0 |
|---|---|---|---|
| 规格: | 100g | 产品价格: | ¥160.0 |
| 规格: | 500g | 产品价格: | ¥480.0 |
| 有效期 | 5年 |
|---|---|
| 英文名称 | NON-Fat Powdered Milk |
| 储存条件 | 2-25℃ |
| 纯度 | Purity≥99.0% |
| 外观(性状) | 白色或类白色粉末 |
用于制备微生物培养基;配制ELISA、western等实验的封闭液
相关文献:
《Effect of 1α, 25-dihydroxyvitamin D3 on the osteogenic differentiation of human periodontal ligament stem cells and the underlying regulatory mechanism》 作者:Yawen Ji, Panpan Zhang ,Yixiao Xing ,Linglu Jia ,Yunpeng Zhang, Tingting Jia ,Xuan Wu ,Bin Zhao, Xin Xu 期刊:International Journal of Molecular Medicine 影响因子:2.928 PMID:30365053
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文献和实验after the final wash, as above. 3.Pipette 100 µl of 5% skim milk/PBS/0.005% thimerasol (added to retard bacterial growth) to each well. Incubate the plate, covered with Parafilm, at 37°C for 2 hours to overnight. It is convenient during this incubation step
on that material are also appropriate. MRS media Electroporation Buffer:(0.4 M sucrose, 1 mM MgCl2, 5 mM Kh2PO4; PH 6) Milk Medium: (0.2 M sucrose, 5% skim milk, 0.1% yeast extract, 1% Casamino Acids, 25mM MgCl2) Erythromycin
Blocking of unwanted non-specific staining
4- Incubate with skim milk 5 in TBS-T for 10-30 min. (Miller RT et al, Appl. Immunohist & Molecular Morphology, 71(1): 63-65, 1999) NOTE: skim milk may contain weak proteases which will be useful to further unmask antigens, but may reduce immunostaining
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