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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human FCGRT & B2M Heterodimer Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/10.00 µg/20.00 µg/50.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥27600.0 |
|---|---|---|---|
| 规格: | 10.00 µg | 产品价格: | ¥2050.0 |
| 规格: | 20.00 µg | 产品价格: | ¥3220.0 |
| 规格: | 50.00 µg | 产品价格: | ¥5170.0 |
| 规格: | 100.00 µg | 产品价格: | ¥4730.0 |
蛋白名称:Human FCGRT & B2M Heterodimer Protein (His Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (P55899-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61770-1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1. Measured by its ability to bind human IgG1 (Cat: 10702-HNAC) in a functional ELISA. 2. Labeled biotin to FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag) bound to Conbercept was 0.1 μM.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ala 24 & Ile 21
蛋白分子量:The recombinant heterodimer of human FCGRT/B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.8+11.7) kDa. The apparent molecular mass of human FCGRT/B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions.
蛋白NP号:P55899-1 & P61770-1
蛋白氨基酸序列:Met1-Ser297 & Met1-Met119
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Goulet, DR; et al. Towards a combinatorial approach for the prediction of immunoglobulin G half-life and clearance. Drug metabolism and disposition: the biological fate of chemicals, PMID: 30232177
3, Wada, R; et al. Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms. mAbs, PMID: 30466347
4, Noy-Porat, T; et al. Fc-Independent Protection from SARS-CoV-2 Infection by Recombinant Human Monoclonal Antibodies. Antibodies (Basel, Switzerland), PMID: 34842604
5, Zhou, F; et al. A Novel Dual-Fc Bispecific Antibody with Enhanced Fc Effector Function. Biochemistry, PMID: 38426700
6, Zhou, C; et al. Preclinical investigations and a first-in-human phase 1a trial of JS00
7, a novel anti-CTLA-4 antibody, in patients with advanced solid tumors. Experimental hematology & oncology, PMID: 39354625
7, Lv, Y; et al. An antibody against human interleukin-2 showing minimal binding to neonatal Fc receptor: Potent anti-tumor activity and reduced systemic toxicity in mice. European journal of pharmacology, PMID: 39615864
8, Kim, DS; et al. Engineering IgG antibodies for intracellular targeting and drug delivery. Journal of controlled release : official journal of the Controlled Release Society, PMID: 40222416
9, Lau-Kilby, A; et al. Development and preclinical characterization of AMG 329: a human antibody neutralizing FLT3 ligand. mAbs, PMID: 40624796
HIS 标签蛋白纯化效果不理想,宝宝心里苦呀。今天,小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的 HIS 标签蛋白。该问题主要可以分为以下两个方面: 1、HIS 标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高,容易导致蛋白炭化;功率过低,蛋白释放不出来。 建议 改变超声功率,同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素,如:金属离子螫合剂、强还原剂、过高的咪唑浓度。同时,优化缓冲
蛋白标签(protein tag)是指利用DNA体外重组技术,与目的蛋白一起融合表达的一种多肽或者蛋白,以便于目的蛋白的表达、检测、示踪和纯化等。随着技术的不断发展,研究人员相继开发出了具有各种不同功能的蛋白标签。 一、 新型蛋白标签 蛋白标签应用极为广泛,但仍有两个问题不容忽视:(1)蛋白标签以DNA形式编码,它需要转录并翻译成蛋白后才能发挥作用,而这种作用方式本身就是一种缺陷。例如,常用的荧光蛋白标签,在显微镜下观察时,其显像不如人工合成的荧光基团好,但是这种人工合成的荧
[资源][原创] 蛋白标签纯化技术——CBP(钙调蛋白结合肽)
缓冲液中能够特异性的被钙调蛋白树脂捕捉吸附,并且在中性环境中能够被2 mM EGTA洗脱,这与6组氨酸亲合标签纯化体系相比反应条件要温和许多。仅有4-kDa 大小的CBP tag 与26-kDa GST 标签相比对蛋白分子的影响非常小。在所表达的蛋白分子上包含一个凝血酶或肠激酶列解位点,依靠它可以非常方便的移除CBP标签。在哺乳动物细胞中表达的标签纯化系统(两步纯化法用于高纯度的要求)Mammalian TAP System本系统同时用到了streptavidin binding peptide
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