FCGRT & B2M Heterodimer重组蛋白|Recombinant Human FCGRT & B2M Heterodimer Protein (His Tag)
- ￥2050 - 27600
- Sino Biological
- 北京
- CT009-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human FCGRT & B2M Heterodimer Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/10.00 µg/20.00 µg/50.00 µg/100.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥27600.0 |
|---|---|---|---|
| 规格： | 10.00 µg | 产品价格： | ￥2050.0 |
| 规格： | 20.00 µg | 产品价格： | ￥3220.0 |
| 规格： | 50.00 µg | 产品价格： | ￥5170.0 |
| 规格： | 100.00 µg | 产品价格： | ￥4730.0 |
蛋白名称：Human FCGRT & B2M Heterodimer Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (P55899-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61770-1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its ability to bind human IgG1 (Cat: 10702-HNAC) in a functional ELISA. 2. Labeled biotin to FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag) bound to Conbercept was 0.1 μM.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ala 24 & Ile 21
蛋白分子量：The recombinant heterodimer of human FCGRT/B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.8+11.7) kDa. The apparent molecular mass of human FCGRT/B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions.
蛋白NP号：P55899-1 & P61770-1
蛋白氨基酸序列：Met1-Ser297 & Met1-Met119
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Goulet DR, et al. Towards a combinatorial approach for the prediction of immunoglobulin G half-life and clearance.Drug metabolism and disposition: the biological fate of chemicals, PubMed ID: 30232177
3, Wada R, et al. Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms.mAbs, PubMed ID: 30466347
4, Noy-Porat T, et al. Fc-Independent Protection from SARS-CoV-2 Infection by Recombinant Human Monoclonal Antibodies.Antibodies (Basel, Switzerland), PubMed ID: 34842604
5, Zhou F, et al. A Novel Dual-Fc Bispecific Antibody with Enhanced Fc Effector Function.Biochemistry, PubMed ID: 38426700
X-100或脱氧胆酸钠）和低浓度变性剂（2mol/L尿素或盐酸胍等）洗涤除去脂类和膜蛋白，这一步很重要，否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂，如8mol/L尿素、6~8mol/L盐酸胍，通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差，异氰盐酸胍最强；去污剂，如SDS[7]，可以破坏蛋白内的疏水键，可以增溶几乎所有的蛋白，但由于无法彻底去除而不允许用在制药行业中；酸，如70%甲酸[9]，可以破坏
剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择： 尿素和盐酸胍属中强度变性剂，易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用，所需浓度尿素8-10M，盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱，溶解度为70-90%，尿素在作用时间较长或温度较高时会裂解形成氰酸盐，对重组蛋白质的氨
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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