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- PEPROTECH
- 中国/美国
- xy-114Hu01
- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Fc Fragment Of IgG Receptor Transporter Alpha (FcgRT)
- 库存：
1000
- 供应商：
上海信裕
IgA-Fc断片受体转运蛋白α(FcgRT)重组蛋白
Recombinant Fc Fragment Of IgG Receptor Transporter Alpha (FcgRT)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | xy114Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 90% |
| UOM | 50ug |
| Predicted Molecular Mass | 41.0kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
IgA-Fc断片受体转运蛋白α(FcgRT)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
AESHLSL LYHLTAVSSP APGTPAFWVS GWLGPQQYLS YNSLRGEAEP CGAWVWENQV SWYWEKETTD LRIKEKLFLE AFKALGGKGP YTLQGLLGCE LGPDNTSVPT AKFALNGEEF MNFDLKQGTW GGDWPEALAI SQRWQQQDKA ANKELTFLLF SCPHRLREHL ERGRGNLEWK EPPSMRLKAR PSSPGFSVLT CSAFSFYPPE LQLRFLRNGL AAGTGQGDFG PNSDGSFHAS SSLTVKSGDE HHYCCIVQHA GLAQPLRVEL ESPAKSSVLV VGIVIGVLLL TAAAVGGALL WRRMRSGLPA PWISLRGDDT GVLLPTPGEA QDADLKDVNV IPATA
AESHLSL LYHLTAVSSP APGTPAFWVS GWLGPQQYLS YNSLRGEAEP CGAWVWENQV SWYWEKETTD LRIKEKLFLE AFKALGGKGP YTLQGLLGCE LGPDNTSVPT AKFALNGEEF MNFDLKQGTW GGDWPEALAI SQRWQQQDKA ANKELTFLLF SCPHRLREHL ERGRGNLEWK EPPSMRLKAR PSSPGFSVLT CSAFSFYPPE LQLRFLRNGL AAGTGQGDFG PNSDGSFHAS SSLTVKSGDE HHYCCIVQHA GLAQPLRVEL ESPAKSSVLV VGIVIGVLLL TAAAVGGALL WRRMRSGLPA PWISLRGDDT GVLLPTPGEA QDADLKDVNV IPATA
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
IgA-Fc断片受体转运蛋白α(FcgRT)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
IgA-Fc断片受体转运蛋白α(FcgRT)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
IgA-Fc断片受体转运蛋白α(FcgRT)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
的血凝块的超薄切片中发现，Hp与“O”型RBC（后来证实按Lewis分型属Leb型”，及Hp与胃粘膜上皮细胞之间的关系，在高倍电镜下所见非常相似。这些特异的亲和现象很可能暗示着类似的粘附机制。1993年有人发现Hp特异地粘附于人胃粘膜上皮细胞受到人类初乳中SCIgA（一种携带高度多变的N－和O－连接的单糖链的糖蛋白）的抑制。当这种SC-IgA被墨角藻糖酶（α－L－fuco-sidase）降解后，抑制作用即降低，若用唾液酸（sialic acid）处理（曾有人报道Hp表达唾液酸特异的凝集素），并不
，有些激素是许多细胞必不可少的，如胰岛素。 (3）酶抑制剂：培养贴壁生长的细胞，需要用胰酶消化传代，在无血清培养基中必不可少须含酶抑制剂，以终止酶的消化作用，达到保护细胞的目的。最常用的是大豆胰酶；抑制剂。 (4）结合蛋白和转运蛋白：常见如转铁蛋白和牛血清白蛋白。牛血清白蛋白的添加比较大，可增加培养基的粘度，保护细胞免受机械损伤。许多旋转式培养的无血清培养基都含有牛血清白蛋白。 (5)微量元素：硒是最常见的。 二、使用方法:目前，血清仍是动物细胞培养中最基本的的添加物，尤其是在原代
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IgA-Fc断片受体转运蛋白α(FcgRT)重组蛋白
¥1 - 3980
