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说明:
Nano-Glo® Live Cell Assay System 是一种用于检测活细胞内NanoBiT® 或NanoLuc® 生物发光的单次添加、非细胞裂解性的检测试剂。使用Nano-Glo® LCS Dilution Buffer 稀释Nano-Glo®Live Cell Substrate 配制成5X 的Nano-Glo® Live CellReagent,该5X 储备液可直接添加至细胞培养基中。底物和缓冲液都经过优化具有增强的稳定性。无论是否有血清存在,Nano-Glo®Live Cell Reagent 都可减少自发光,增强检测低水平NanoBiT® 或NanoLuc® 发光信号的灵敏度。Nano-Glo® Live Cell Assay System可用于检测自定义时间点的发光信号或者连续检测发光信号达2 小
时,同时不损害细胞活力。
储存条件:
Nano-Glo® LCS Dilution Buffer 可能融化,请储存于室温。其它组分储存于-30℃到-10℃。
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文献和实验Live-Cell Migration and Adhesion Turnover Assays
Fluorescence microscopy has revolutionized the way live-cell imaging is achieved. At the same time, it is also potentially harmful to a living specimen. Therefore, the specimen must be monitored for viability and health
Functional Screening with a Live Cell Imaging-Based Random Cell Migration Assay
of the players/pathways is required. For this purpose, we developed a cell migration assay based on automatic high-throughput microscopy screen. This approach allows for screening of hundreds of genes, e.g., those encoding various kinases and phosphatases
Methods to Measure Nuclear Export of -Catenin Using Fixed and Live Cell Assays
and regulation in and out of the nucleus. In this chapter, we outline a number of assays to measure the nuclear export of β-catenin in fixed and live cells.
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