Methods to Measure Nuclear Export of -Catenin Using Fixed and Live Cell Assays

β-Catenin is a multifunctional protein which is overexpressed in several types of cancers. The subcellular location of β-catenin at the membrane junctions or in the nucleus determines its function in cell adhesion and transcription activation, respectively. The aberrant localization of β-catenin in the nucleus activates transcription of a number of genes involved in cancer progression. Our laboratory studies intracellular trafficking of β-catenin with particular emphasis on its movement and regulation in and out of the nucleus. In this chapter, we outline a number of assays to measure the nuclear export of β-catenin in fixed and live cells.