NFkB-p65(Phospho-Ser536) Antib

Product Name：
NFkB-p65(Phospho-Ser536) Antibody

Host Species：
Rabbit

Clonality：
Polyclonal

Purification：
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Applications：
WB IHC IF

Species Reactivity：
Hu Ms Rt

Specificity：
The antibody detects endogenous level of NF-kB p65 only when phosphorylated at serine 536.

Immunogen Type：
Peptide-KLH

Immunogen Description：
Peptide sequence around phosphorylation site of serine 536 (F-S-S(p)-I-A) derived from Human NFkB-p65.

Target Name：
NFkB-p65

Modification：
Phospho

Alternative Names：
NFKB3; RELA; TF65; Transcription factor p65; p65

Accession No. ：
Swiss-Prot: Q04206
NCBI Protein: NP_001138610.1

Concentration：
1.0mg/ml

Formulation：
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Images：

Western blot analysis of extracts from HepG2 and HL60 cells untreated or treated with TNF using NFkB-p65(Phospho-Ser536) Antibody #11014.


Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser536) Antibody #11014 (left) or the same antibody preincubated with blocking peptide #51014 (right).


Immunofluorescence staining of methanol-fixed Hela cells using NFkB-p65(Phospho-Ser536) Antibody #11014.


Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser536) Antibody #11014.


Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using NFκB-p65 (Phospho-Ser536) Antibody #11014.


Immunofluorescence staining of methanol-fixed MEF cells using NFκB-p65 (Phospho-Ser536) Antibody #11014.