- 询价
- Signalway Antibody
- t504
- USA
- 2025年07月10日
- WB
- Mouse
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- 详细信息
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- 文献和实验
- 技术资料
- 抗体英文名：
Myc-Tag Mouse Monoclonal Antibody
- 靶点：
Myc-Tag
- 浓度：
1.0mg/ml
- 应用范围：
WB
- 宿主：
Mouse
- 保质期：
Store at 4°C for short term use. Store at -20°C for long term preservation. Avoid freezing and thawing repeatly.
- 克隆性：
单克隆
- 保存条件：
Store at -20℃
- 形态：
liquid
- 免疫原：
Peptide sequence around aa. 410-419 (EQKLISEEDL) derived from human c-Myc.
9E10
Isotype
IgG1
Purification
Antibodies were produced from mice ascites by injecting mice with a monoclonal cell line which was fusioned by mouse spleen and SP2/0 myeloma cell. Spleen cells were isolated from mice by immunizing with synthetic peptide and KLH conjugates.
Applications
WB
Specificity
The mouse mAb detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected cells.
Immunogen Type
Peptide
Immunogen Description
Peptide sequence around aa. 410-419 (EQKLISEEDL) derived from human c-Myc.
Target Name
Myc-Tag
Accession No.
NCBI Protein: NP_002458.2
Alternative Names：
N/A
Concentration
1.0mg/ml
Formulation
Supplied in mice ascites
Application Details
Western blotting: 1:5000~1:10000
Background
Epitope tags are artificial epitopes useful for the labeling and detection of proteins. Epitope tags - short amino acid sequences are 'fused' to the N- or C-terminus of the protein.
Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.
Images
Western blot analysis of extracts from 293 cells transfected with Myc-CDK2 using Myc-Tag mouse mAb #T504.
产品详细信息请点击：Myc-Tag Mouse Monoclonal Antibody
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Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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