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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Golden Syrian Hamster
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX100539
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, IP, FISH, IHC
- 浓度:
0.11 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
IP/MS validation was supported by references (PMID:30377373)
- 抗体英文名:
PCNA antibody
- 抗体名:
PCNA 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PCNA antibody (GTX100539) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: PCNA protein stained by PCNA antibody (GTX100539) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.
PCNA antibody detects PCNA protein at nucleus in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon .
PCNA antibody (GTX100539) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PCNA antibody detects PCNA protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
PCNA antibody (GTX100539) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
PCNA antibody detects PCNA protein at nucleus in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
PCNA antibody (GTX100539) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PCNA antibody detects PCNA protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (GTX100539) diluted at 1:2500.
Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 μg of PCBP2 antibody (GTX100539).
Western blot analysis was performed using PCBP2 antibody (GTX100539).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PCNA antibody (GTX100539) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
PCNA antibody detects PCNA protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
12% SDS-PAGE
PCNA antibody (GTX100539) dilution: 1:2500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PCNA protein stained by PCNA antibody (GTX100539) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
PCNA antibody detects PCNA protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
PCNA antibody (GTX100539) dilution: 1:2500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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May EW et al., Pharmacol Res 2014 (PMID:25242635)
Tomas J et al., ISME J 2014 (PMID:25012905)
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