- ￥2262
- LSM Bio
- 进口
- PAab06215
- 2025年07月17日
- ELISA,IF,IP,WB,IHC,FC
- E. coli - derived recombinant protein
- Human,Mouse ,Rat,Zebrafish
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
proliferating cell nuclear antigen
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
Polyclonal antibody
- 标记物：
Non-conjugated
- 适应物种：
Human,Mouse ,Rat,Zebrafish
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
Q9WVH4
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA,IF,IP,WB,IHC,FC
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
proliferating cell nuclear antigen
- 抗体英文名：
anti-PCNA antibody,PCNA antibody
- 抗体名：
anti-PCNA 抗体,PCNA 抗体
- 规格：
100μl
PCNA抗体| PCNA antibody
货号 PAab06215
蛋白别名 Proliferating cell nuclear antigen antibody,Cyclin
蛋白介绍
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA.
产品描述
anti-PCNA antibody is a Rabbit Polyclonal antibody againstPCNA..
建议稀释比例
WB:1:1000-1:10000 IP:1:500-1:5000 IHC:1:200-1:1000 IF:1:20-1:200
IHC
Immunohistochemistry of paraffin-embedded human stomach cancer using PAab06215(PCNA antibody) at dilution of 1:50
Western blot
A431 cells were subjected to SDS PAGE followed by western blot with PAab06215(PCNA antibody) at dilution of 1:1000(本抗体仅供体外科研用途，不可用于临床诊断!) <"">
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文献和实验In Vitro PCNA Modification Assays
Modification of the replicative sliding clamp, PCNA, by monoubiquitin, polyubiquitin, and SUMO contributes to the processing of DNA damage during replication. In order to investigate the properties of the relevant conjugation enzymes
Detection of PCNA Modifications in Saccharomyces cerevisiae
PCNA modifications by members of the ubiquitin family are associated with a range of different transactions during replication of damaged and undamaged DNA. This chapter describes detailed protocols for the detection and isolation
Flow Cytometric Detection of Proliferation-Associated Antigens, PCNA and Ki-67
The rapid evolution of monoclonal antibody (MAb) technology has resulted in an ever-growing repertoire of proteins becoming accessible for study using immunohistochemical techniques. Nuclear antigens represent a class of proteins
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