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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
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| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 2X RNA Loading Dye | ||||
| SM1831 | 100 µl | 1.00 ml | SM1831 |
- Features
-
- 条带窄且亮度一致。
- 条带大小和含量容易记忆。
- 条带浓度预先设置好,可用于RNA条带的粗略定量。
- 配套提供2X RNA Loading Dye。
- -20°C可稳定保存6个月。
- Applications
-
- 变性胶和非变性胶中RNA分子大小鉴定和定量。
- Northern杂交。
RiboRuler™ Low Range RNA Ladder 由7条分子量大小为100-1000 b的单链RNA转录子组成的混合物
RiboRuler™ Low Range RNA ladders由层析纯化的转录子组成,无NTPs和RNA降解产物污染。每个RNA转录子的浓度均由分光光度计测定。为了减少使用时的污染机率,建议将Ladders分装后保存。
RiboRuler™ Low Range RNA ladders 可用 于T4 Polynucleotide Kinase (#EK0031)催化的末端标记(见操作方法)和Northern杂交等应用。
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文献和实验electrophoresis at 100 V until the bromophenol blue dye has migrated two-thirds the gel length. 6) Stain the RNA ladder with: i. 0.5 μg/ml EtBr solution for 10 minutes and detain the gel in deionized water for 10 minutes OR
Eukaryotic messenger RNA (mRNA) can be separated from the other RNA species in a total RNA preparation by affinity chromatography by virtue of the presence of a polyadenylic acid “tail,” 20–25 bases long, at the 3′ end of the molecule
containing100µl (6-7×108 beads/ml) of Dynabeads™ M-280 Streptaviden (Figure 1b).Pulldown (3), RNA-biotin specific Incubate the elute/Dynabead™ slurry at 4°C for 15 minutes on an orbital shaker followed by capture with a magnetic bead separator. The captured
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