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reporter dye (hexachlorofluorescein, R) on one end and a dark quencher (Q)
on the other end。它的序列经过精心优化与各种核酸酶反应; 它包含与单链内切酶反应的结构域,单链核酸外切酶和双链核酸酶。完整,基板几乎没有荧光。当被DNA酶切割时,底物发出粉红色(536nm或UV)激发,556nm发射)。DNaseAlert测试快速而简单。实验室表面或液体试剂用不到一个小时就可以测试和验证为“无核酸酶”或“污染”。为了速度和易用性,一个简单的基于tube的分析可以直接在测试现场进行。或者,定量可以获得荧光结果(比色皿或微量滴定板形式)并用于记录GLP或制造需求的核酸酶测试。
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文献和实验a substrate that is cleaved by the 5" nuclease activity of Taq DNA polymerase when the enzyme extends from an upstream primer into the region of the probe. This dependence on polymerization ensures that cleavage of the probe occurs only if the target sequence
Propagation, Quantification, Detection, and Storage of West Nile Virus
MultiScribe reverse transcriptase Nuclease‐free H 2 O (use for all steps requiring H 2 O
Using Molecular Beacons for Sensitive Fluorescence Assays of the Enzymatic Cleavage of Nucleic Acids
A novel method for DNA enzymatic cleavage assays using molecular beacons (MBs) as the substrate for nuclease is described. An MB is a hairpin-shaped DNA probe that is labeled with a fluorescent dye at one end and a quencher at the other end










