- 询价
- Thermo scientific -fermentas
- SM0223
- 2025年10月22日
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|
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 6X DNA Loading Dye | ||||
| SM0223 | 100 applic. (0.1 µg/µl) | 1.00 ml | SM0223 |
- Features
-
- 大分子DNA大小鉴定和粗略定量。
- 条带窄。
- 即用型包装与6X DNA Loading Dye预先混合，可直接上样(上样前需加热处 理)和室温保存。
- 配套提供样本DNA上样染料。
pUC19 DNA/MspI (HpaII) markers提供即用型的包装。其预先与6X DNA Loading Dye混合，可直接上样琼脂糖凝胶和聚丙烯酰胺凝胶电泳。
备注
- ** 标记的条带在聚丙烯酰胺凝胶中会产生非正常的条带。
- 短片段(斜体)在常规电泳时无法显示，其片段长度是根据DNA序列由计算机预测出来的。
-20°C可保存更长时间。
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文献和实验Methylation analyis using restriction enzyme digestion
. Since in eukaryotic DNA (or actually in mammalian DNA) only cytosine in CG context can be methylated, the restriction enzymes with CG sequences within their restriction sites come in question. Two classical enzyme pairs are HpaII - MspI (CCGG) and SmaI - XmaI (CCCGGG
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
by vortexing. 21. Centrifuge at maximum speed ($13,000 x g) for 5 minutes at room temperature to collect precipitated DNA. Carefully discard supernatant. 22. Add 600 μL of 70% ethanol and mix throughly by vortexing for 30 seconds. 23
Methylation analyis using restriction
µg of DNA overnight with a first enzyme,cutting out the fragment of interest in combination with HpaII,and another 10 µg with first enzyme in combination with MspI.Reaction volume should be about 50 µl. Inactivate the enzymes by heating (look
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