- 询价
- Thermo scientific -fermentas
- SM1833
- 2025年10月23日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 2X RNA Loading Dye | ||||
| SM1833 | 50 applic. | 1.00 ml | SM1833 |
- Features
-
- 条带窄且亮度一致。
- 条带大小和含量容易记忆。
- 条带浓度预先设置好，可用于RNA条带的粗略定量。
- 配套提供2X RNA Loading Dye。
- -20°C可稳定保存6个月。
- Applications
-
- 变性胶和非变性胶中RNA分子大小鉴定和定量。
- Northern杂交。
RiboRuler™ Low Range RNA Ladder, ready-to-use, 由7条分子量大小为100-1000 b的单链RNA转录子组成的混合物
RiboRuler™ Low Range RNA ladders, ready-to-use, 由层析纯化的转录子组成，无NTPs和RNA降解产物污染。每个RNA转录子的浓度均由分光光度计测定。为了减少使用时的污染机率，建议将Ladders分装后保存。
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文献和实验electrophoresis at 100 V until the bromophenol blue dye has migrated two-thirds the gel length. 6) Stain the RNA ladder with: i. 0.5 μg/ml EtBr solution for 10 minutes and detain the gel in deionized water for 10 minutes OR
(1 ). Oligo(dT)-cellulose is used routinely for homemade affinity columns, but a range of ready-made products is now available from a number of suppliers (e.g., Hybond-mAp, Amersham, Arlington Heights, IL; PolyATract™, Promega, Madison, WI). Oligo(dT
Isolation of RNA from Difficult Tissues
ToTALLY RNA™ Kit). Note that many of these techniques can be used with other RNA isolation protocols as well. Heart and Skeletal Muscle: Fibrous Tissues For fibrous tissues such as rat and mouse heart and skeletal muscle, the most difficult step
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