- 询价
- Thermo scientific -fermentas
- EN0201
- 2025年11月05日
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- 技术资料
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|
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 10X Reaction Buffer | ||||
| EN0201 | 100 u (5 u/µl) | 1.00 ml | EN0201 | |
| EN0202 | 500 u (5 u/µl) | 1.00 ml | EN0202 |
- Applications
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- 第二链cDNA合成前除去mRNA (1)。
- RT-PCR和qRT-PCR：第一链cDNA合成后除去RNA模板。
- 通过与oligo(dT)杂交后除去mRNA中的poly(A)序列(2)。
- 位点特异性切割RNA (3)。
- 体外聚腺苷酸化反应产物研究(4)。
酶活性分析混合物：20 mM Tris-HCl (pH 7.8), 40 mM KCl, 8 mM MgCl2 , 1 mM DTT, 24 µM �3 H]-poly(A)・poly(dT), 0.03 mg/ml BSA, 4% (v/v)甘油。
25 mM HEPES-KOH (pH 8.0), 50 mM KCl, 0.1 mM �DTA, 1 mM DTT, 0.1 mg/ml BSA和50% (v/v)甘油。
- 抑制剂：金属螯合剂、SH-封闭试剂。
- 失活：65°C加热10分钟。
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文献和实验RNase H: Specificity, Mechanisms of Action, and Antiviral Target
The Ribonuclease (RNase) H is one of the four enzymes encoded by all retroviruses, including HIV. Its main activity is the hydrolysis of the RNA moiety in RNA–DNA hybrids. The RNase H ribonuclease is essential in the retroviral life cycle
Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold
scaffold, using RNase H and two DNA oligonucleotides. After cleavage, we show that the RNA of interest can be isolated in a one-step purification. This method has, in particular, applications in structural investigations of RNA.
Question 1.What are the differences among RNase H, RNase A, RNase B and RNase C? 2.In your cDNA kits, RNase H is added in the second strand reaction to produce more nicked RNA as primers for DNA synthesis. In this repect
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