- 询价
- Thermo scientific -fermentas
- EN0181
- 2025年11月04日
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大量
|
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| EN0181 | 8000 u (300 u/µl) | EN0181 |
- Applications
-
- 水解无细胞抽提液中的核酸(1)。
- RNA测序(2)。
- 染色质结构研究(3)。
- 蛋白折叠和结构-功能研究模型(4, 5)。
酶活性分析混合物：100 mM sodium-glycine (pH 8.6), 10 mM CaCl2 , 4.5 mM牛胸腺DNA。
20 mM Tris-HCl (pH 7.6), 50 mM NaCl和50% (v/v)甘油。
- 抑制剂：金属螯合剂、脱氧胸苷3’5’-二磷酸。
- 失活：加入EGTA或EDTA。
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文献和实验Assay for the Micrococcal Nuclease
Method: Essentially that described by Heins et al. (1966) based upon the release of acid soluble oligonucleotides following nuclease digestion of DNA. One unit corresponds to a change in optical density of 1.0 at 260 nm at 37°C and pH
Measuring Changes in Chromatin Using Micrococcal Nuclease
This chapter documents a simple protocol to identify the nucleosome positioning of any given genes. The procedure includes partitioning 200-bp DNA fragments constituting the nucleosomal core region by micrococcal nuclease digestion
Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease
of digestion of DNA by micrococcal nuclease when naked DNA is compared to the same DNA bound by a nucleosome. Curve fitting to a function that describes the amount of DNA remaining following a series of digestions over a broad range of micrococcal nuclease
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