- 询价
- Cell Signaling Technology
- 3900
- USA
- 2025年09月22日
- 免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）,染色质免疫沉淀分析（ChIP）
- 兔
- ,
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- 详细信息
- 技术资料
- 宿主：
兔
- 适应物种：
,
- 应用范围：
免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）,染色质免疫沉淀分析（ChIP）
- 级别：
详见MSDS文件
- 保存条件：
-20°c
- 抗体英文名：
Rabbit (DA1E) mAb IgG XP Isotype Control
- 是否单克隆：
1
- 抗原来源：
/
- 保质期：
详见说明书
- 库存：
大量
- 供应商：
CST
- 抗原：
/
- 规格：
250 ug /<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /250 ug /<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥询价 | |
|---|---|---|---|
| 规格： | 250 ug | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 250 ug | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Complementary Reagents
Rabbit (DA1E) mAb IgG XP® Isotype Control #3900
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IHC-P IF-IC F ChIP | Endogenous | Rabbit IgG |
Applications Key: IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry ChIP=Chromatin IP
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3900:
- ChIP Agarose , ChIP Magnetic , Flow , IHC / Paraffin , Immunofluorescence , Immunoprecipitation
Specificity / Sensitivity
Rabbit (DA1E) mAb IgG XP® Isotype Control is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.
Source / Purification
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (right).
Flow Cytometry
Flow cytometric analysis of K-562 cells, untreated (green) or STI-treated (blue), using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb #9314 compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (red).
IF-IC
Confocal immunofluorescent analysis of A549 cells using β-Tubulin (9F3) Rabbit mAb #2128 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-F
Confocal immunofluorescent analysis of normal rat cerebellum using DARPP-32 (19A3) Rabbit mAb #2306 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-P
Confocal immunofluorescent analysis of paraffin-embedded human colon adenocarcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Chromatin IP
Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and the indicated antibodies. Purified DNA was analyzed by quantitative real-time PCR, using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The relative abundance of each DNA sequence enriched by Rabbit (DA1E) mAb IgG XP® Isotype Control (red) is compared to the amount of the same DNA sequence enriched by the histone H3-specific immunoprecipitations (blue).
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 2975 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)
- 2985 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate)
- 4340 Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate)
- 3452 Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
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