- 询价
- Cell Signaling Technology
- 4903
- USA
- 2025年09月14日
- western blot,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 兔
- 人,驴
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- 详细信息
- 文献和实验
- 技术资料
- 适应物种：
人,驴
- 应用范围：
western blot,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 供应商：
CST
- 抗原：
/
- 抗原来源：
/
- 保质期：
详见说明书
- 库存：
大量
- 宿主：
兔
- 抗体英文名：
Nanog (D73G4) XP Rabbit mAb
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (8 western blots)/100 ul (20 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /40 ul (8 western blots)/100 ul (20 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥询价 | |
|---|---|---|---|
| 规格： | 40 ul (8 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (20 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 40 ul (8 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (20 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Lineage Markers
Nanog (D73G4) XP® Rabbit mAb #4903
PhosphoSitePlus ® protein, site, and accession data: NANOG
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H (Mk) | Endogenous | 42 | Rabbit IgG |
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4903:
- Flow , IHC / Paraffin , Immunofluorescence , Western Blotting
Specificity / Sensitivity
Nanog (D73G4) XP® Rabbit mAb detects endogenous levels of total nanog protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human nanog protein.
Western Blotting
Western blot analysis of extracts from NCCIT, NTERA-2 and iPS cells using Nanog (D73G4) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (D73G4) XP® Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Nanog (D73G4) XP® Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog (D73G4) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Background
Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).
- Kim, J. et al. (2008) Cell 132, 1049-61.
- Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
- Yu, J. et al. (2007) Science 318, 1917-20.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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- 5677 Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated)
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For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验3A7)XP® Rabbit mAb#9145 检测 p-Stat3 的表达，使用不同的产品进行显色。与竞争者的 DAB 产生的信号相比，SignalStain® DABSubstrateKit 的显色强度更佳。CST 对实验细节进行了步步优化，抗体说明书也是为实验成功保驾护航的必备指南：注：使用 PLK1(208 G4)Rabbit mAb #4513 检测人结肠肿瘤中 PLK1 的表达，在经过辅助试剂的步步优化后发现，使用一抗稀释液#8112 稀释抗体，聚合物型二抗#8114 进行检测，DAB 显色
肺癌病人的诊断和个性化治疗提供了有效的筛查工具。同样，以ROS1融合蛋白抗体（克隆号D4D6，货号#3287）为基础的IHC检测技术与断裂FISH方法相比，在非小细胞肺癌上IHC检测的准确度达到100%【5】。如此，我们期待检测ROS1融合变异的临床诊断用IHC试剂盒不久将成功推出。如要了解更多有关ALK和ROS1抗体的验证数据，请点击：ALK (D5F3) XP®Rabbit mAb #3633 和 ROS1 (D4D6) Rabbit mAb #3287。 本文
。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
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