Phospho-p38 MAPK (Thr180/Tyr18

Product Pathways - MAPK Signaling

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Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP^® Rabbit mAb #4511

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P IF-IC F	H M R Mk Sc (Hm) (C) (Z) (B) (Pg)	Endogenous	43	Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  Z=Zebrafish  B=Bovine  Pg=Pig  Sc=S. cerevisiae
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

4511:

Flow , IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting

Specificity / Sensitivity

Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP^® Rabbit mAb detects endogenous levels of p38 MAPK only when phosphorylated at Thr180 and Tyr182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK.

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
2. Han, J. et al. (1994) Science 265, 808-811.
3. Lee, J.C. et al. (1994) Nature 372, 739-746.
4. Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
5. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
6. Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
7. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
8. Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
9. Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
10. Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.

Application References

• Bruchas, M.R. et al. (2011) Neuron 71, 498-511. Applications: IF-P (paraffin)
• Won, A.P. et al. (2011) Proc Natl Acad Sci U S A 108, 9809-14. Applications: Western Blotting
• Rohani, M.G. et al. (2010) BMC Immunol 11, 53. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

Companion Products

• 1170 Phospho-p38 MAPK (Thr180/Tyr182) Blocking Peptide
• 9913 Phospho-p38 MAPK Pathway Sampler Kit
• 9213 p38 MAPK Control Cell Extracts
• 9215 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb
• 2371 p38α MAPK (7D6) Rabbit mAb
• 9217 p38α MAPK (5F11) Mouse mAb
• 9228 p38α MAPK (L53F8) Mouse mAb
• 2339 p38β MAPK (C28C2) Rabbit mAb
• 2308 p38δ MAPK (10A8) Rabbit mAb
• 9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb
• 4631 Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb
• 2307 p38γ MAPK Antibody
• 8690 p38 MAPK (D13E1) XP^® Rabbit mAb
• 9212 p38 MAPK Antibody
• 9218 p38α MAPK Antibody
• 9214 p38δ MAPK Antibody
• 9210 PhosphoPlus^® p38 MAPK (Thr180/Tyr182) Antibody Kit
• 9219 Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb (Sepharose Bead Conjugate)
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)
• 5425 Normal Goat Serum
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)

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For Research Use Only. Not For Use In Diagnostic Procedures.