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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human R-Spondin 1 / RSPO1 Protein
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/5.00 µg/20.00 µg/100.00 µg/500.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥25880.0 |
|---|---|---|---|
| 规格: | 5.00 µg | 产品价格: | ¥580.0 |
| 规格: | 20.00 µg | 产品价格: | ¥1780.0 |
| 规格: | 100.00 µg | 产品价格: | ¥4980.0 |
| 规格: | 500.00 µg | 产品价格: | ¥14480.0 |
重组人 R-Spondin 1 / RSPO1 蛋白(产品说明)
蛋白名称:Human R-Spondin 1 / RSPO1 Protein
蛋白构建:A DNA sequence encoding the human RSPO1 (NP_001033722.1) (Met1-Ala263) was expressed and purified.
表达宿主:CHO Stable Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC
蛋白活性:Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 10-80 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
蛋白内毒素:< 10 EU per mg protein.
预测N端:Ser 21
蛋白分子量:The recombinant human RSPO1 consists of 243 amino acids and has a predicted molecular mass of 26.8 kDa. As a result of glycosylation, the apparent molecular mass of it is approximately 40 and 31 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001033722.1
蛋白氨基酸序列:Met 1-Ala 263
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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- 作者
- 内容
- 询问日期
文献和实验2, Yao, Y; et al. Patient-Derived Organoids Predict Chemoradiation Responses of Locally Advanced Rectal Cancer. Cell stem cell, PMID: 31761724
3, Li, Y; et al. Bach2 Deficiency Promotes Intestinal Epithelial Regeneration by Accelerating DNA Repair in Intestinal Stem Cells. Stem cell reports, PMID: 33382975
4, Hao, M; et al. Patient-Derived Organoid Model in the Prediction of Chemotherapeutic Drug Response in Colorectal Cancer. ACS biomaterials science & engineering, PMID: 35696669
5, Feng, J; et al. Feedback activation of EGFR/wild-type RAS signaling axis limits KRASG12D inhibitor efficacy in KRASG12D-mutated colorectal cancer. Oncogene, PMID: 37020035
6, Rao, X; et al. Unveiling Epigenetic Vulnerabilities in Triple-Negative Breast Cancer through 3D Organoid Drug Screening. Pharmaceuticals (Basel, Switzerland), PMID: 38399440
7, Cui, Y; et al. The combination of breast cancer PDO and mini-PDX platform for drug screening and individualized treatment. Journal of cellular and molecular medicine, PMID: 38722288
8, Yang, C; et al. Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy. Cancer cell, PMID: 39515328
9, Cheng, X; et al. Characterizing the effects of triclosan and triclocarban on the intestinal epithelial homeostasis using small intestinal organoids. Journal of hazardous materials, PMID: 39244982
10, Xu, X; et al. Comprehensive dissection of rectal cancer organoids in responses to chemoradiation. Cell reports. Medicine, PMID: 41045935
11, Wasdin, PT; et al. Generation of antigen-specific paired-chain antibodies using large language models. Cell, PMID: 41192421 1
2, Zhao, Y; et al. Extracellular cyclophilin a binding to CD146 is critical for Th17 cell differentiation in rheumatoid arthritis. Molecular therapy : the journal of the American Society of Gene Therapy, PMID: 41137392
Nature 解读:David R. Liu 等通过体内碱基编辑实现治疗小鼠Hutchinson-Gilford早衰综合征
Hutchinson-Gilford 早衰综合症(HGPS 或早衰症)通常是由编码核纤层蛋白 A 的基因 LMNA 中 C・G 基因突变为 T・A(c.1824 C> T;p.G608G)引起的。这种突变导致 RNA 错剪,产生早老素(progerin)。早老素是一种有毒蛋白,可引起快速衰老,导致早衰儿童的寿命大约只有 14 岁。尽管治疗早衰的策略例如全面抑制蛋白质法尼基化(farnesylation)可为患者带来益处,但尚没有报道可以直接逆转 HGPS 突变的方法。2021 年 1 月
质芯片检测。此外,肽芯片或基于质谱的方法[ 20,21] 也可以用于这方面。 我们在此介绍基于蛋白质芯片技术的蛋白磷酸化筛选方法和高通量确定蛋白激酶底物的方法。我们已成功地用这种筛选工具鉴定大麦酪蛋白激酶 2α ( CK 2α) 和不同的拟南芥丝裂原活化蛋白(MAP) 激酶 [23] 的新靶标。我们这个方法使用本书第 28 章详细描述的植物蛋白质芯片(见第 28 章)。在放射性【 γ33 磷】三磷酸腺苷存在的条件下,用可溶和具有活性的激酶孵育芯片。通过磷屏成像仪或 X 射线胶片检测到的放射性信号,对可能
杆菌的包涵体可采用离心收获。 洗涤 包涵体常用的洗涤试剂为中性去垢剂和还原剂。包涵体中主要由重组蛋白组成,同时也含有一些外膜蛋白(Omp C、Omp F、Omp A)、16S 和 23S、5S的r RNA、质粒 DNA 和其它杂质。去除这些杂质,可采用去垢剂 Triton X-100、脱氧胆酸盐和低浓度的变性剂等对包涵体充分洗涤。包涵体洗涤的洁净程度,对于后续的纯化步骤十分重要,包涵体纯度高,可以大大降低后续的纯化压力。但是,变性剂尿素的使用应该注意的是大肠杆菌外膜蛋白Omp T(37 kD
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