β-Tubulin Antibody

价格询价

品牌Cell Signaling Technology
货号
2146
产地USA
更新日期2025年08月09日

应用范围W, IHC-P, IF-IC, F
宿主Rabbit
适应物种H,M,R,Mk,Z,B,X

美国CST中国公司(丁香通商铺)

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详细信息
文献和实验
技术资料

抗体英文名：
β-Tubulin Antibody
抗原：
synthetic peptide corresponding to the sequence of human β-tubulin
应用范围：
W, IHC-P, IF-IC, F
宿主：
Rabbit
级别：
详见MSDS文件
保质期：
详见说明书
适应物种：
H,M,R,Mk,Z,B,X
供应商：
CST
库存：
大量
是否单克隆：
2
保存条件：
-20°c
规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey X=Xenopus Z=Zebrafish B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IHC-P IF-IC F	H M R Mk Z B (X)	Endogenous	55	Rabbit

Protocols	2146: Flow , IHC / Paraffin , Immunofluorescence , Western Blotting
Specificity / Sensitivity	The β-Tubulin Antibody detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin.
Source / Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human β-tubulin. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting Western blot analysis of extracts from CAD and C6 cells using β-Tubulin Antibody. Western Blotting Western blot analysis of recombinant alpha-tubulin and beta-tubulin GST-fusion proteins and extracts from CAD cells using α-Tubulin Antibody #2144(left), β-Tubulin Antibody (middle) and GST Antibody #2622 (right). IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb #2128 in the presence of control peptide (left) or β-Tubulin Blocking Peptide (right). IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using β-Tubulin Antibody. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using β-Tubulin Antibody. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human MALToma, using β-Tubulin Antibody. Flow Cytometry Flow cytometric analysis of C6 cells, using β-Tubulin Antibody (blue) compared to a nonspecific negative control antibody (red). IF-IC Confocal microscopic images of NIH/3T3 cells showing cytoskeletal stain with β-Tubulin Antibody (A) compared to an isotype control (B).
Background	The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). Westermann, S. and Weber, K. (2003) Nat. Rev. Mol. Cell Biol. 4, 938 -947.
Application References	Xie, Y.B. et al. (2009) Nucleic Acids Res 37, 4100-15. Applications: Western Blotting Widenmaier, S.B. et al. (2009) J Biol Chem 284, 30372-82. Applications: Western Blotting Xie, Y.B. et al. (2009) J Biol Chem 284, 28762-74. Applications: Western Blotting Beharry, Z. et al. (2009) Mol Cancer Ther 8, 1473-83. Applications: Western Blotting Eliopoulos, N. et al. (2010) Am J Physiol Renal Physiol 299, F1288-98. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products	2144 α-Tubulin Antibody 2148 α/β-Tubulin Antibody 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody 7074 Anti-rabbit IgG, HRP-linked Antibody 7720 Prestained Protein Marker, Broad Range (Premixed Format) 7727 Biotinylated Protein Ladder Detection Pack 7003 20X LumiGLO^® Reagent and 20X Peroxide 1032 β-Tubulin Blocking Peptide 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit) 8112 SignalStain^® Antibody Diluent For Research Use Only. Not For Use In Diagnostic Procedures.