- 询价
- Cell Signaling Technology
- 2317
- USA
- 2025年07月12日
- western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 小鼠
- 人,小鼠,大鼠,驴,其他
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- 文献和实验
- 技术资料
- 抗体英文名：
S6 Ribosomal Protein (54D2) Mouse mAb
- 抗原：
/
- 应用范围：
western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 宿主：
小鼠
- 供应商：
CST
- 适应物种：
人,小鼠,大鼠,驴,其他
- 抗原来源：
/
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Translational Control
S6 Ribosomal Protein (54D2) Mouse mAb #2317
PhosphoSitePlus ® protein, site, and accession data: S6
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M R Mk Dm | Endogenous | 32 | Mouse IgG1 |
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2317:
- Flow , IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting
Specificity / Sensitivity
S6 Ribosomal Protein (54D2) Mouse mAb detects endogenous levels of total S6 ribosomal protein independent of phosphorylation.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant fusion protein corresponding to full-length human S6 ribosomal protein.
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using S6 Ribosomal Protein (54D2) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using S6 Ribosomal Protein (54D2) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using S6 Ribosomal Protein (54D2) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded LNCaP cells, using S6 Ribosomal Protein (54D2) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using S6 Ribosomal Protein (54D2) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells, using S6 Ribosomal Protein (54D2) Mouse mAb (blue) compared to a nonspecifc negative control antibody (red).
IF-IC
Confocal immunofluorescent images of HeLa cells labeled with S6 Ribosomal Protein (54D2) Mouse mAb (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
- Dufner, A. and Thomas, G. (1999) Exp. Cell Res. 253, 100-109.
- Peterson, R.T. and Schreiber, S.L. (1998) Curr. Biol. 8, R248-R250.
- Jefferies, H.B. et al. (1997) EMBO J. 16, 3693-3704.
- Ferrari, S. et al. (1991) J. Biol. Chem. 266, 22770-22775.
- Flotow, H. and Thomas, G. (1992) J. Biol. Chem. 267, 3074-3078.
Application References
- De Raedt, T. et al. (2011) Cancer Cell 20, 400-13. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 4858 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb
- 2211 Phospho-S6 Ribosomal Protein (Ser235/236) Antibody
- 2215 Phospho-S6 Ribosomal Protein (Ser240/244) Antibody
- 2217 S6 Ribosomal Protein (5G10) Rabbit mAb
- 9203 p70 S6 Kinase Control Cell Extracts
- 8101 SignalSlide® Phospho-Akt (Ser473) IHC Controls
- 8112 SignalStain® Antibody Diluent
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
构成核糖体的蛋白质。大肠杆菌核糖体蛋白的初级结构均被确定。大肠杆菌核糖体的 30S亚基含 S1 — S21 共 21种蛋白质， 50S亚基含 L1— L34共 34种蛋白质。这些蛋白质已被全部分离纯化。分子量约 1万到 3万。除 S6 、 L7 、 L12 之外全是碱性蛋白质。这些蛋白质是免疫学上独立的蛋白质，只有 L7 、 L1 2 显示出相互交叉反应。已知 L7 与 L1 2 是同一蛋白质， L7 的 N末端被乙酰化。已经确定了几种蛋白的一级结构。机能已经
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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