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- SL100489-CACO
- 2026年02月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
一年
- 英文名：
GenJet™ In Vitro DNA Tranfection Reagent for Caco-2 Cell
- 库存：
现货 大量
- 供应商：
济南美中清水湾生物科技有限公司
- 保存条件：
4°C
- 规格：
毫升/支
GenJet™ DNA In Vitro Tranfection Reagent for Caco-2 Cell is pre-optimized for transfecting Caco-2 cells. The Caco-2 cell line is an immortalized line of heterogeneous human epithelial colorectal adenocarcinoma cells, developed by the Sloan-Kettering Institute for Cancer Research through research conducted by Dr. Jorgen Fogh. The Caco-2 cell line is widely used with in vitro assays to predict the absorption rate of candidate drug compounds across the intestinal epithelial cell barrier. The assay requires that drug absorption rates be determined 21 days after Caco-2 cell seeding to allow for monolayer formation and cell differentiation. Caco-2 cells may also refer to a cell monolayer absorption model. Cell-based functional assays, such as the Caco-2 drug transport model for assessing intestinal transport, are extremely valuable for screening lead compounds in drug discovery.
Refer to the following optimal transfection conditions for maximal transfection efficiency on Caco-2 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium 5 Hours After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
~90% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes 48 hours EGFP pEGFP-N3 (CMV promoter) 65% |
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on Caco-2 cell with GenJet™ reagent. Caco-2ela cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 48 hours post transfection
Data Sheet
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文献和实验Permeability Assessment Using 5-day Cultured Caco-2 Cell Monolayers
where inserts are coated with collagen protein and the culturing cell media contains sodium butyrate to modify differentiation and growth properties of the cell line. Protocol conditions for the accelerated 5 day Caco-2 and conventional 21 day Caco-2 assays
Use of Caco-2 Cell Monolayers to Study Drug Absorption and Metabolism
The Caco-2 cell culture model is used to determine the absorption potentials of drug candidates and the transport and metabolism mechanisms of drugs and dietary chemicals. The Food and Drug Administration (FDA) recognized the model
Acetaldehyde-induced Barrier Disruption and Paracellular Permeability in Caco-2 Cell Monolayer
. We showed that acetaldehyde disrupts intestinal epithelial tight junctions and increases paracellular permeability to endotoxins in Caco-2 cell monolayer, the extensively studied model of the differentiated intestinal epithelium. The mechanisms involved
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