- ¥1560
- SignaGen
- 美国
- SL100489-CaS
- 2026年02月07日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
一年
- 英文名：
GenJet™ In Vitro DNA Tranfection Reagent for Ca Ski Cells
- 库存：
现货 大量
- 供应商：
济南美中清水湾生物科技有限公司
- 保存条件：
4°C
- 规格：
毫升/支
GenJet™ DNA In Vitro Tranfection Reagent for Ca Ski Cell is pre-optimized for transfecting Ca Ski Cells, human cervix epidermoid carcinoma cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on Ca Ski cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
80% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, 18~24 Hours After Transfection 48 hours EGFP pEGFP-N3 (CMV promoter) 88% |
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
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文献和实验(2002) Origin, history, and uses of oat ( Avena sativa ) and wheat ( Triticum aestivum). Course Agronomy 212, Iowa State University, Department of Agronomy, Iowa. 7. http ： //interactive, usask. ca/Ski/agriculture/crops/cereals
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
Support Protocol 2: Preparation of Genomic DNA Plugs from Unicellular Nucleated Cells Alternate Protocol 1: Module‐Specific PCR for Determination of the Number of RP‐C4‐CYP21‐TNX (RCCX) Modules or Total C4 Gene Dosage
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