GenJet™ In Vitro DNA Tranfection Reagent for SHEP Cell
库存:
现货 大量
供应商:
济南美中清水湾生物科技有限公司
保存条件:
4°C
Description
GenJet™ DNA In Vitro Tranfection Reagent for SHEP is pre-optimized for transfecting SHEP cells. This human neuroblastoma cell line was cloned from a single primary tumor. Unlike other neuroblastoma cell, SHEP cells does not produce IGF-II, expresses five-fold fewer IGF-IRs than SH-SY5Y and dies in serum-free media or following exposure to metabolic stressors.
Refer to the following optimal transfection conditions for maximal transfection efficiency on SHEP cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency