GenJet™ In Vitro DNA Tranfection Reagent for Epithelial Cell
库存:
现货 大量
供应商:
济南美中清水湾生物科技有限公司
保存条件:
4°C
规格:
毫升/支
Description
GenJet™ DNA In Vitro Tranfection Reagent for epithelial cell is pre-optimized for epithelial cells. By cross-linking a peptide ligand, this pre-optimized GenJet™ reagent specifically binds epithelial cells and give superior efficiency on epithelial cells.
Refer to the following optimal transfection conditions for maximal transfection efficiency on epithelial cells. GenJet™ reagent, 1.0 ml, is sufficient for 600 to 1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.
Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency
~80% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, ~5 Hours After Transfection 48 hours
EGFP pEGFP-N3 (CMV promoter) 65%
Storage Condition Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A comparison showing superior efficiency of the pre-optimized GenJet (Ver. II) on normal human colonic epithelial cells. Transfection was performed per the vendors' protocol. 0.5 µg of GFP DNA (pEGFP-N3) per well in 24-well plate was delivered to normal human colonic epithelial cells with different transfection methods. The GFP positive cells were counted under a Nikon 2000 Eclipse fluorescence microscope 24 hours post transfection.