- ¥1590
- SignaGen
- 美国
- SL100489-EPI
- 2026年04月24日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保质期：
一年
- 英文名：
GenJet™ In Vitro DNA Tranfection Reagent for Epithelial Cell
- 库存：
现货 大量
- 供应商：
济南美中清水湾生物科技有限公司
- 保存条件：
4°C
- 规格：
毫升/支
GenJet™ DNA In Vitro Tranfection Reagent for epithelial cell is pre-optimized for epithelial cells. By cross-linking a peptide ligand, this pre-optimized GenJet™ reagent specifically binds epithelial cells and give superior efficiency on epithelial cells.
Refer to the following optimal transfection conditions for maximal transfection efficiency on epithelial cells. GenJet™ reagent, 1.0 ml, is sufficient for 600 to 1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
~80% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, ~5 Hours After Transfection 48 hours EGFP pEGFP-N3 (CMV promoter) 65% |
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A comparison showing superior efficiency of the pre-optimized GenJet (Ver. II) on normal human colonic epithelial cells. Transfection was performed per the vendors' protocol. 0.5 µg of GFP DNA (pEGFP-N3) per well in 24-well plate was delivered to normal human colonic epithelial cells with different transfection methods. The GFP positive cells were counted under a Nikon 2000 Eclipse fluorescence microscope 24 hours post transfection.
Data Sheet
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Integrin receptors mediate the adhesion of cells to the extracellular matrix and to other cells. They play a significant role in a variety of biological functions
Thymic Epithelial Cell Culture
, antigen presentation, and direct interaction with T-lymphocyte precursors added to the cultures, as well as for establishment of epithelial cell lines.
The establishment of liver epithelial cell lines or strains from newborn or adult rat liver has been reported by many investigators (1 -6 ). The cells of these lines are smaller in size and morphologically more simple than hepatocytes
技术资料暂无技术资料 索取技术资料
